Expression of PDX-1 is reduced in pancreatic islets from pups of rat dams fed a low protein diet during gestation and lactation

dc.creatorArantes, VC
dc.creatorTeixeira, VPA
dc.creatorReis, MAB
dc.creatorLatorraca, MQ
dc.creatorLeite, AR
dc.creatorCarneiro, EM
dc.creatorYamada, AT
dc.creatorBoschero, AC
dc.date2002
dc.dateOCT
dc.date2014-11-18T04:00:22Z
dc.date2015-11-26T16:51:05Z
dc.date2014-11-18T04:00:22Z
dc.date2015-11-26T16:51:05Z
dc.date.accessioned2018-03-28T23:37:53Z
dc.date.available2018-03-28T23:37:53Z
dc.identifierJournal Of Nutrition. Amer Inst Nutrition, v. 132, n. 10, n. 3030, n. 3035, 2002.
dc.identifier0022-3166
dc.identifierWOS:000178447600009
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/66491
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/66491
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/66491
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1275897
dc.descriptionIntrauterine and early postnatal malnutrition has profound consequences on fetal and postnatal development in both humans and animals. In addition, low birth weight has been reported to be associated with impaired insulin secretion, insulin resistance and diminished area of pancreatic islets. Because the transcription factor pancreatic and duodenal homeobox 1 (PDX-1) is important for the maintenance of B-cell physiology, PDX-1 expression and islet area were assessed in neonatal rats of dams fed low (6%) or normal (17%) protein diets during pregnancy. PDX-1 protein and mRNA levels, as well as insulin secretion and islet area, were measured after 28 d of life in normal, low protein and recovered rats whose dams consumed a normal protein diet after delivery. Insulin secretion by isolated islets in response to 2.8 and 16.7 mmol glucose/L was reduced in 28-d-old low protein rats compared with the control (P < 0.05). At birth and after 28 d of life, the islet area and PDX-1 protein expression were also reduced (P < 0.05). In contrast, PDX-1 mRNA levels in islets from 28-d-old low protein rats were not different from control rats. PDX-1 protein expression in pancreatic islets, the area of islets and insulin secretion were restored in recovered rats, whereas PDX-1 mRNA levels were higher than in normal rats (P < 0.05). These results suggest a link among diminished PDX-1 protein expression, a reduction in islet area and impaired insulin secretion in low protein rats. The reintroduction of a normal diet early in life restored islet area and cell physiology.
dc.description132
dc.description10
dc.description3030
dc.description3035
dc.languageen
dc.publisherAmer Inst Nutrition
dc.publisherBethesda
dc.publisherEUA
dc.relationJournal Of Nutrition
dc.relationJ. Nutr.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectpancreatic islets
dc.subjectlow protein diet
dc.subjectinsulin secretion
dc.subjectislet size
dc.subjectrats
dc.subjectInduced Insulin-secretion
dc.subjectP70 S6 Kinase
dc.subjectBeta-cells
dc.subjectGene-expression
dc.subjectMessenger-rna
dc.subjectBinding-activity
dc.subjectRin-5f Cells
dc.subjectGlucose
dc.subjectTranscription
dc.subjectFetal
dc.titleExpression of PDX-1 is reduced in pancreatic islets from pups of rat dams fed a low protein diet during gestation and lactation
dc.typeArtículos de revistas


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