An assessment was made of the hydrodynamic behavior and organic matter removal of a fixed bed anaerobic sequencing batch reactor with liquid-phase circulation, containing 0.5cm polyurethane foam-cubes. The hydrodynamic study was performed without biomass and with superficial rates ranging from 0.16 to 0.80cm/s, employing continuous operation to quantify the flow behavior in the bed and discontinuous operation to quantify mixing time. In the continuous mode the bed showed a piston flow behavior. In the discontinuous operation mode the mixing time obtained in all conditions may be considered negligible when compared to the total cycle time to be employed in the assay with biomass. In the assay regarding organic matter removal, 8h cycles were employed in sequencing batch mode using anaerobic biomass. The reactor was maintained at 30 1C, treated glucose-based synthetic wastewater with a concentration of approximately 500mgCOD/L. Reactor performance was assessed for the condition without liquid circulation as well as at a superficial circulation rate of 0.19cm/s. The reactor presented better performance and stability for the circulation condition, achieving efficiencies as high as 95% organic matter removal.304188+