Artículos de revistas
Isolation and characterization of novel neurotoxins from south American rattlesnake Crotalus durissus terrificus: Optimization of chromatographic procedure
Registro en:
Protein And Peptide Letters. Bentham Science Publ Ltd, v. 7, n. 6, n. 381, n. 388, 2000.
0929-8665
WOS:000165246800005
Autor
Toyama, MH
Leite, GB
Rodrigues-Simioni, L
Hernandez, OSS
Novello, JC
Marangoni, S
Institución
Resumen
Molecular exclusion HPLC chromatography on a Protein Pak SW 300 column and reversed phase HPLC on a mu -Bondapack C18 column were used to purify four novel neurotoxins from the crotoxin complex, and five crotoxin subunits (PLA(2) and crotapotin) as well as two crotamine isoforms. A single preparative reversed phase HPLC steps yielded a major basic myotoxin followed by the subunits of crotoxin (three PLA(2) isoforms and two crotapotin isoforms). This modified procedure may be useful for the rapid, large-scale purification of toxins from Crotalid venoms. 7 6 381 388