Artículos de revistas
Phosphoprotein levels, MAPK activities and NF kappa B expression are affected by fisetin
Registro en:
Journal Of Enzyme Inhibition And Medicinal Chemistry. Taylor & Francis Ltd, v. 22, n. 4, n. 439, n. 444, 2007.
1475-6366
WOS:000248489700008
10.1080/14756360601162063
Autor
De Sousa, RRR
Souza Queiroz, KC
Santos Souza, AC
Gurgueira, SA
Augusto, AC
Miranda, MA
Peppelenbosch, MP
Ferreira, CV
Aoyama, H
Institución
Resumen
Flavonoids, polyphenolic phytochemicals, are ubiquitous in plants and are commonly present in the human diet. They may exert diverse beneficial effects, including antioxidant and anticarcinogenic activities. The present study was designed to evaluate three biomolecules that play important roles in the apoptotic process: mitogen-activated protein kinases, protein phosphatases and NFkB, using HL60 cells treated with fisetin as an experimental model. Our results demonstrated that cells treated with fisetin presented high expression of NFkB, activation of MAPK p38 and an increase of phosphoprotein levels; inhibition of enzymes involved in redox status maintenance were also observed. Our findings reinforce the hypothesis that fisetin is likely to exert beneficial and/or toxic actions on cells not through its potential as antioxidant but rather through its modulation of protein kinase and phosphatase signaling cascades. Additionally, our results also indicate that the cellular effects of fisetin will ultimately depend on the cell type and on the extent to which they associate with the cells, either by interactions at the membrane or by uptake into the cytosol. 22 4 439 444