Artículos de revistas
Isomaltulose production by free cells of Serratia plymuthica in a batch process
Registro en:
Food Chemistry. Elsevier Sci Ltd, v. 120, n. 3, n. 789, n. 793, 2010.
0308-8146
WOS:000275014200022
10.1016/j.foodchem.2009.11.011
Autor
Kawaguti, HY
Sato, HH
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Free cells of Serratia plymuthica were used to convert sucrose into isomaltulose and trehalulose reducing Sugars. The effects of substrate concentration and temperature were observed in a batch process with flasks shaken at 150 rpm. The experimental design and response surface methodology analysis indicated that the conversion parameters had significant influence (p < 0.05) on sucrose conversion, and that a valid model was obtained after an analysis of variance (F(model) = 10.48 > F(charted) = 5.79) to obtain a response surface and isocurve. Conversion was favoured when a 30% sucrose solution and temperature of 25 degrees C were used, which resulted in a high conversion into isomaltulose - over 70% - and 7-8% trehalulose. Small amounts of glucose (5-7%) and fructose (5-8%) were formed in the reaction medium. (C) 2009 Elsevier Ltd. All rights reserved. 120 3 789 793 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)