Artículos de revistas
Quantifying loss of CD34(+) cells collected by apheresis after processing for freezing and post-thaw
Registro en:
Transfusion And Apheresis Science. Pergamon-elsevier Science Ltd, v. 48, n. 2, n. 241, n. 246, 2013.
1473-0502
WOS:000319538300047
10.1016/j.transci.2013.01.017
Autor
Castelhano, MV
Reis-Alves, SC
Vigorito, AC
Rocha, FF
Pereira-Cunha, FG
De Souza, CA
Lorand-Metze, I
Institución
Resumen
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Introduction: CD34(+) cells collected for autologous bone marrow transplantation (BMT) are usually quantified in the apheresis product after collection, but the necessity to repeat these measures post-thaw is controversial. Methods: We examined the loss of CD34(+) cells after collection, preparation for freezing and post-thaw in apheresis products collected for BMT. Results: Median number of CD34(+) cells collected per unit was 1.61 x 10(6)/kg, viability: 97-100%. This number decreased to 1.38 x 10(6)/kg, viability: 96-100% before freezing and was 1.17 x 10(6)/kg post-thaw. Viability decreased to 86-98%. The relative loss of viable PBHPC showed an inverse correlation with the ratio "CD34(+) cells/total nucleated cells" (r = -0.45; p = <0.0005). This relative loss was largest in patients with Hodgkin's lymphoma. Conclusion: Cryopreservation and thawing of PBHPCs in leukapheresis products provokes a small but significant stem cell loss. So, quantification of viable CD34(+) cells post-thaw is important, especially in poorly mobilizing patients. Besides, the ratio "CD34(+) cells/total nucleated cells" after leukapheresis is an important parameter for prediction of neutrophil recovery after BMT. (c) 2013 Elsevier Ltd. All rights reserved. 48 2 241 246 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)