Artículos de revistas
Production and characterization of chitosan microparticles containing papain for controlled release applications
Registro en:
Powder Technology. Elsevier Science Sa, v. 205, n. 41699, n. 65, n. 70, 2011.
0032-5910
WOS:000284787900008
10.1016/j.powtec.2010.08.066
Autor
Vasconcellos, FC
Goulart, GAS
Beppu, MM
Institución
Resumen
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The production and modification of chitosan microparticles using crosslinking agents and papain were evaluated for controlled release applications. Chitosan microparticles were produced and crosslinked with sodium tripolyphosphate (TPP) 10% (w/v) solution or glutaraldehyde (GLU) 0.75% (w/w), with subsequent papain sorption. Microparticles were characterized by Fourier transformed infrared spectroscopy (FTIR) for chemical modifications, scanning electron microscopy (SEM) for morphology and X-ray diffraction (XRD) for crystallographic analysis. Chemical composition and the thermal stability of the material were characterized by thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). It was observed that the presence of TPP and papain resulted in a decrease of the stability of the chitosan matrix. Papain release rates from the microparticles were also conducted in vitro. The amount of released papain in phosphate buffer (pH = 7.4) was analyzed with UV-spectroscopy, showing release profiles of enzymatic activity ranging from 0.006 to 0.011 mu mol.min(-1). The results indicate that both chitosan-TPP-papain and chitosan-GLU-papain microparticles can successfully be used for systems that aim at a controlled release of papain with potential use in the biomedical area. (C) 2010 Elsevier B.V. All rights reserved. 205 41699 65 70 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) FAEPEX-UNICAMP Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)