Artículos de revistas
Microsatellite loci for an endemic stingless bee Melipona seminigra merrillae (Apidae, Meliponini) from Amazon
Registro en:
Conservation Genetics Resources. Springer, v. 1, n. 1, n. 487, n. 490, 2009.
1877-7252
WOS:000207922400109
10.1007/s12686-009-9113-9
Autor
Francini, IB
Sforca, DA
Sousa, ACB
Campos, T
Cidade, FW
Zucchi, MI
Souza, AP
Nunes-Silva, CG
Carvalho-Zilse, GA
Institución
Resumen
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Melipona seminigra merrillae is one of the main pollinators of Central Amazon native flora. These stingless bees are reared as economic alternative and for forest conservation purposes. Nine polymorphic microsatellite markers were characterized in 44 genotypes of M. seminigra merrillae from the Meliponary of the Instituto Nacional de Pesquisas da Amazonia, INPA (3 degrees 05.838'S 059 degrees 59.103'W), Manaus, Brazil. The number of alleles observed for each locus ranged from 3 to 8, with an average of 3.7 alleles per locus. The polymorphism information content (PIC) varied between 0.21 and 0.81 (average 0.50) and the discriminating power (D) ranged from 0.59 to 0.98 (average 0.77). The observed heterozygosity (H(o)) and the expected heterozygosity (H(e)) were 0.04-0.68 and 0.19-0.84, respectively. A cross-amplification test in three Melipona species suggested potential transferability of these microsatellites. These microsatellite markers should be useful for characterizing natural populations and specie conservation. 1 1 487 490 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundacao de Amparo a Pesquisa do Estado do Amazonas (FAPEAM) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)