Determination of salicylate in blood serum using an amperometric biosensor based on salicylate hydroxylase immobilized in a polypyrrole-glutaraldehyde matrix

dc.creatorJunior, LR
dc.creatorNeto, GD
dc.creatorFernandes, JR
dc.creatorKubota, LT
dc.date2000
dc.date38777
dc.date2014-12-02T16:26:58Z
dc.date2015-11-26T16:33:36Z
dc.date2014-12-02T16:26:58Z
dc.date2015-11-26T16:33:36Z
dc.date.accessioned2018-03-28T23:15:32Z
dc.date.available2018-03-28T23:15:32Z
dc.identifierTalanta. Elsevier Science Bv, v. 51, n. 3, n. 547, n. 557, 2000.
dc.identifier0039-9140
dc.identifierWOS:000085712300014
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/61420
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/61420
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/61420
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1270914
dc.descriptionThe use of an amperometric biosensor for the salicylate determination in blood serum is described. The biosensor is based on salicylate hydroxylase (EC 1.14.13.1) electropolymerized onto a glassy carbon-working electrode with polypyrrole and glutaraldehyde, to improve the biosensor lifetime. The hexacyanoferrate: (II) was also incorporated to work as a redox mediator to minimize possible interferences. The salicylate is enzymatically converted to catechol, which is monitored amperometrically by its electrooxidation at + 0.170 V versus SCE (saturated calomel electrode). Salicylate determination was carried out maintaining the ratio between beta-NADH and salicylate at 4:1 (30 degrees C). The amperometric response of the biosensor was linearly proportional to the salicylate concentration between 2.3 x 10(-6) and 1.4 x 10(-5) mol l(-1), in 0.1 mol l(-1) phosphate buffer (pH 7.8), containing 0.1 mol l(-1) KCl and 5.0 x 10(-4) mol l(-1) Na(2)H(2)EDTA, as supporting electrolyte. The recovery studies, in the presence of several interfering compounds, showed recoveries between 96.4 and 104.8%. The useful lifetime of the biosensor in the concentration range evaluated was at least 40 days, in continuous use. Blood serum samples analyzed by this biosensor showed a good correlation compared to the spectrophotometric method (Trinder) used as reference, presenting relative deviations lower than 7.0%. (C) 2000 Elsevier Science B.V. All rights reserved.
dc.description51
dc.description3
dc.description547
dc.description557
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationTalanta
dc.relationTalanta
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectsalicylate determination
dc.subjectserum samples
dc.subjectpolypyrrole
dc.subjectsalicylate hydroxylase
dc.subjectCarbon Paste Electrodes
dc.subjectFlow-injection Analysis
dc.subjectLiquid-chromatography
dc.subjectEnzyme Electrode
dc.subjectPharmaceutical Preparations
dc.subjectAcetylsalicylic-acid
dc.subjectSelective Electrode
dc.subjectAspirin
dc.subjectSystem
dc.subjectPlasma
dc.titleDetermination of salicylate in blood serum using an amperometric biosensor based on salicylate hydroxylase immobilized in a polypyrrole-glutaraldehyde matrix
dc.typeArtículos de revistas


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