Artículos de revistas
Automated measurement of mouse freezing behavior and its use for quantitative trait locus analysis of contextual fear conditioning in (BALB/cJ x C57BL/6J)F-2 mice
Registro en:
Learning & Memory. Cold Spring Harbor Lab Press, v. 5, n. 41763, n. 391, n. 403, 1998.
1072-0502
WOS:000076812700012
Autor
Valentinuzzi, VS
Kolker, DE
Vitaterna, MH
Shimomura, K
Whiteley, A
Low-Zeddies, S
Turek, FW
Ferrari, EAM
Paylor, R
Takahashi, JS
Institución
Resumen
The most commonly measured mouse behavior in fear conditioning tests is freezing. A technical Limitation, particularly for genetic studies, is the method of direct observation used for quantifying this response, with the potential for bias or inconsistencies. We report the use of a computerized method based on latency between photobeam interruption measures as a reliable scoring criterion in mice. The different computer measures obtained during contextual fear conditioning tests showed high correlations with hand-scored freezing; r values ranged from 0.87 to 0.94. Previously reported strain differences between C57BL/6J and DBA/2J in context-dependent fear conditioning were also detected by the computer-based system. In addition, the use of computer-scored freezing of 199 (BALB/cJ x C57BL/6J)F-2 mice enabled us to detect a suggestive gender-dependent chromosomal locus for contextual fear conditioning on distal chromosome 8 by QTL analysis. Automation of freeze scoring would significantly increase efficiency and reliability of this learning and memory test. o TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE AGOSTO DE 2015. 5 41763 391 403