Artículos de revistas
Increasing mouse embryonic fibroblast cells adhesion on superhydrophilic vertically aligned carbon nanotube films
Registro en:
Materials Science & Engineering C-materials For Biological Applications. Elsevier Science Bv, v. 31, n. 7, n. 1505, n. 1511, 2011.
0928-4931
WOS:000295953900037
10.1016/j.msec.2011.06.007
Autor
Lobo, AO
Marciano, FR
Ramos, SC
Machado, MM
Corat, EJ
Corat, MAF
Institución
Resumen
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) We have analyzed the adhesion of mouse embryonic fibroblasts (MEFs) genetically modified by green fluorescence protein (GFP) gene cultured on vertically-aligned carbon nanotubes (VACNTs) after 6 days. The VACNTs films grown on Ti were obtained by microwave plasma chemical vapor deposition process using Fe catalyst and submitted to an oxygen plasma treatment, for 2 min, at 400 V and 80 mTorr, to convert them to superhydrophilic. Cellular adhesion and morphology were analyzed by scanning electron, fluorescence microscopy, and thermodynamics analysis. Characterizations of superhydrophilic VACNTs films were evaluated by contact angle and X-Ray Photoelectron Spectroscopy. Differences of crowd adhered cells, as well as their spreading on superhydrophilic VACNTs scaffolds, were evaluated using focal adhesion analysis. This study was the first to demonstrate, in real rime, that the wettability of VACNTs scaffolds might have enhanced and differential adherence patterns to the MEF-GFP on VACNTs substrates. (C) 2011 Elsevier B.V. All rights reserved. 31 7 1505 1511 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) FAPESP [07/00013-4, 06/03525-3]