Artículos de revistas
BIOCHEMICAL-CHARACTERIZATION OF A VASCULAR SMOOTH-MUSCLE CONTRACTING POLYPEPTIDE PURIFIED FROM PHONEUTRIA-NIGRIVENTER (ARMED SPIDER) VENOM
Registro en:
Toxicon. Pergamon-elsevier Science Ltd, v. 31, n. 4, n. 377, n. 384, 1993.
0041-0101
WOS:A1993KY87900002
10.1016/0041-0101(93)90173-G
Autor
MARANGONI, S
BORGES, NCC
MARANGONI, RA
ANTUNES, E
VIEIRA, CA
NOVELLO, JC
DOMONT, GB
GIGLIO, JR
OLIVEIRA, B
DENUCCI, G
Institución
Resumen
Crude Phoneutria nigriventer venom was fractionated by Sephadex, ion-exchange and reverse-phase high performance liquid chromatography. One protein (PNV1) with spasmogenic activity in rabbit vascular smooth muscle was isolated and biochemically characterized. PNV1 has 125 amino acid residues and a calculated mol. wt of 13,899. Special features of the amino acid composition of PNV1 are the presence of two disulfide bridges and the high percentage (27%) of Asx and Glx. The N-terminal amino acid sequence indicates that PNV1 is different from other polypeptides isolated from Phoneutria nigriventer venom. 31 4 377 384