Artículos de revistas
Expression of correctly processed human growth hormone in seeds of transgenic tobacco plants
Registro en:
Molecular Breeding. Kluwer Academic Publ, v. 6, n. 1, n. 47, n. 53, 2000.
1380-3743
WOS:000086760700005
10.1023/A:1009648532210
Autor
Leite, A
Kemper, EL
da Silva, MJ
Luchessi, AD
Siloto, RMP
Bonaccorsi, ED
El-Dorry, HF
Arruda, P
Institución
Resumen
Human growth hormone was expressed in transgenic tobacco seeds using the monocot tissue-specific promoter from sorghum gamma-kafirin seed storage protein gene. During tobacco seed ripening, the expressed hormone was directed to the endoplasmic reticulum by a signal peptide from a Coix prolamin and was secreted into the apoplastic space, where it accounted for 0.16% of total soluble seed protein. The expressed hormone has the same amino acid sequence and receptor-binding properties as the native mature hormone. 6 1 47 53