Artículos de revistas
Acidocalcisomes of Phytomonas francai possess distinct morphological characteristics and contain iron
Registro en:
Microscopy And Microanalysis. Cambridge Univ Press, v. 10, n. 5, n. 647, n. 655, 2004.
1431-9276
WOS:000224197000012
10.1017/S1431927604040887
Autor
Miranda, K
Rodrigues, CO
Hentchel, J
Vercesi, A
Plattner, H
de Souza, W
Docampo, R
Institución
Resumen
Acidocalcisomes are acidic calcium storage compartments described initially in trypanosomatid and apicomplexan parasites, and recently found in other unicellular eukaryotes. The aim of this study was to identify the presence of acidocalcisomes in the plant trypanosomatid Phytomonas francai. Electron-dense organelles of P francai were shown to contain large amounts of oxygen, sodium, magnesium, phosphorus, potassium, calcium, iron, and zinc as determined by X-ray microanalysis, either in situ or when purified using iodixanol gradient centrifugation or by elemental mapping. The presence of iron is not common in other acidocalcisomes. In situ, but not when purified, these organelles showed an elongated shape differing from previously described acidocalcisomes. However, these organelles also possessed a vacuolar H+-pyrophosphatase (V-H+-PPase) as determined by biochemical methods and by immunofluorescence microscopy using antibodies against the enzyme. Together, these results suggest that the electron-dense organelles of P. francai are homologous to the acidocalcisomes described in other trypanosomatids, although with distinct morphology and elemental content. o TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE AGOSTO DE 2015. 10 5 647 655