Artículos de revistas
Effect of mutations within the Cys-rich region of potyvirus helper component-proteinase on self-interaction
Registro en:
Journal Of General Virology. Soc General Microbiology, v. 80, n. 2809, n. 2812, 1999.
0022-1317
WOS:000083466300002
Autor
Urcuqui-Inchima, S
Maia, IG
Drugeon, G
Haenni, AL
Bernardi, F
Institución
Resumen
The first similar to 60 amino acids of the N-terminal part of the potyvirus helper component-proteinase (HC-Pro) include highly conserved residues comprising a Cys-rich region. In the present study, the domain in Potato virus Y sufficient for self-interaction was mapped using the yeast two-hybrid system to the 83 N-terminal amino acids of HC-Pro. Mutations in the conserved His and two Cys residues within the Cys-rich region have a strong debilitating effect on self-interaction when introduced in the full-length HC-Pro, but not when introduced in the N-terminal fragment. 80 11 2809 2812