Antimicrobial activity of an L-amino acid oxidase isolated from Bothrops leucurus snake venom

dc.creatorTorres, AFC
dc.creatorDantas, RT
dc.creatorMenezes, RRPPB
dc.creatorToyama, MH
dc.creatorFilho, ED
dc.creatorOliveira, MF
dc.creatorNogueira, NAP
dc.creatorOliveira, MR
dc.creatorMonteiro, HSA
dc.creatorMartins, AMC
dc.date2010
dc.date2014-11-15T14:38:37Z
dc.date2015-11-26T16:11:29Z
dc.date2014-11-15T14:38:37Z
dc.date2015-11-26T16:11:29Z
dc.date.accessioned2018-03-28T22:59:59Z
dc.date.available2018-03-28T22:59:59Z
dc.identifierJournal Of Venomous Animals And Toxins Including Tropical Diseases. Cevap-unesp, v. 16, n. 4, n. 614, n. 622, 2010.
dc.identifier1678-9199
dc.identifierWOS:000284778000012
dc.identifier10.1590/S1678-91992010000400012
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/77812
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/77812
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/77812
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1267100
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionSome snake venom proteins present enzymatic activities, such as L-amino acid oxidase (LAAO). The aim of this paper was to investigate the effect of Bothrops leucurus total venom (BleuTV) and its fraction LAAO (BleuLAAO) on bacteria, yeast, and promastigote forms of Leishmania amazonensis and Leishmania chagasi, and epimastigote forms of Trypanosoma cruzi. BleuTV was isolated with a Protein Pack 5PW (R) (Waters Corporation, USA), and several fractions were obtained. BleuLAAO was purified to high molecular homogeneity, and its N-terminal amino acid sequence shared a high degree of amino acid conservation with other LAAOs. BleuTV inhibited Staphylococcus aureus growth in a dose-dependent manner, with a minimum inhibitory concentration (MIC) of 25 mu g/mL, which corresponded to its minimum lethal concentration (MLC). BleuTV also inhibited the growth of promastigote forms of L. chagasi and L. amazonensis, with respective IC(50) values of 1.94 mu g/mL and 5.49 mu g/mL. Furthermore, it repressed T. cruzi growth with an IC(50) of 1.14 mu g/mL. However, BleuLAAO did not inhibit the growth of the microorganisms studied and was not toxic to macrophages. BleuTV had low toxicity against macrophages at the concentrations studied. In conclusion, whole venom from Bothrops leucurus inhibited the growth of some microorganisms, including S. aureus, Leishmania sp., and T. cruzi.
dc.description16
dc.description4
dc.description614
dc.description622
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageen
dc.publisherCevap-unesp
dc.publisherBotucatu
dc.publisherBrasil
dc.relationJournal Of Venomous Animals And Toxins Including Tropical Diseases
dc.relationJ. Venom. Anim. Toxins Trop. Dis.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectL-amino oxidase
dc.subjectBothrops leucurus
dc.subjectantimicrobial activity
dc.subjectFunctional-characterization
dc.subjectPlatelet-aggregation
dc.subjectPhospholipase A(2)
dc.subjectAntibacterial
dc.subjectPurification
dc.subjectProteins
dc.subjectToxicity
dc.subjectGrowth
dc.subjectAssay
dc.titleAntimicrobial activity of an L-amino acid oxidase isolated from Bothrops leucurus snake venom
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución