Tissue inhibitor of metalloproteinase-2 (TIMP-2) location in the ventral, lateral, dorsal and anterior lobes of rat prostate by immunohistochemistry

dc.creatorDelella, FK
dc.creatorJustulin, LA
dc.creatorFelisbino, SL
dc.date2007
dc.dateMAR
dc.date2014-11-15T11:24:30Z
dc.date2015-11-26T16:10:46Z
dc.date2014-11-15T11:24:30Z
dc.date2015-11-26T16:10:46Z
dc.date.accessioned2018-03-28T22:59:24Z
dc.date.available2018-03-28T22:59:24Z
dc.identifierCell Biology International. Academic Press Ltd Elsevier Science Ltd, v. 31, n. 3, n. 229, n. 234, 2007.
dc.identifier1065-6995
dc.identifierWOS:000244787800005
dc.identifier10.1016/j.cellbi.2006.10.004
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/77944
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/77944
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/77944
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1266957
dc.descriptionMatrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) play a major role in extracellular matrix component degradation in several normal and abnormal tissue situations; they are also found in human seminal plasma. MMPs have been found in rat prostate secretions and are nearly lobe specific in expression pattern. The aim of this study was to evaluate whether TIMP-2, like other semen components, is expressed differently from different rat prostatic lobes. Immunohistochemical staining was performed in both young and adult rat ventral (VP), lateral (LP), dorsal (DP), and anterior (AP) prostatic lobes and confirmed by western blotting. TIMP-2 expression was found in the epithelial cells in the following sequence: LP > AP > DP > VP, in both young and adult rats. In this study, 100% of adult LP presented histological signs of prostatitis, where TIMP-2 immunostaining was positive in normal epithelium even with intraluminal neutrophils, but was reduced or absent in the epithelium with intraepithelial leukocytes or with periductal stroma disorganization associated with mononuclear cell infiltration. However, TIMP-2 expression in LP was not induced by prostatitis, since younger rat LPs were also strongly TIMP-2 positive. The distal and intermediate VP regions were TIMP-2 negative, but the proximal regions were strongly stained. Western blotting results confirmed the high TIMP-2 expression in the LP lobe. Thus, TIMP-2 is expressed differently between the prostatic lobes and is another nearly lobe-specific protein, which plays a role in the regulation of MMP activity in seminal plasma and glandular homeostasis. TIMP-2 is also another regional ductal variation of VP. Further studies should address whether TIMP-2 expression is related to the highest incidence of rat LP prostatitis and adenocarcinoma. (c) 2006 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
dc.description31
dc.description3
dc.description229
dc.description234
dc.languageen
dc.publisherAcademic Press Ltd Elsevier Science Ltd
dc.publisherLondon
dc.publisherInglaterra
dc.relationCell Biology International
dc.relationCell Biol. Int.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectprostate
dc.subjectmetalloproteinase
dc.subjecttissue inhibitor of metalloproteinase
dc.subjectprostatitis
dc.subjectseminal plasma
dc.subjectcollagen fiber
dc.subjectHuman Seminal Plasma
dc.subjectGelatinolytic Proteinase Activities
dc.subjectDuctal System
dc.subjectMatrix Metalloproteinases
dc.subjectExtracellular-matrix
dc.subjectRegional-variation
dc.subjectCancer Development
dc.subjectSecretions
dc.subjectInflammation
dc.subjectCastration
dc.titleTissue inhibitor of metalloproteinase-2 (TIMP-2) location in the ventral, lateral, dorsal and anterior lobes of rat prostate by immunohistochemistry
dc.typeArtículos de revistas


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