The (A)gamma-195 (C -> G) mutation in hereditary persistence of fetal hemoglobin is not associated with activation of a reporter gene in vitro

dc.creatorSchreiber, R
dc.creatorGoncalves, MS
dc.creatorJunqueira, ML
dc.creatorSaad, STO
dc.creatorKrieger, JE
dc.creatorCosta, FF
dc.date2001
dc.dateAPR
dc.date2014-11-15T08:16:23Z
dc.date2015-11-26T16:10:10Z
dc.date2014-11-15T08:16:23Z
dc.date2015-11-26T16:10:10Z
dc.date.accessioned2018-03-28T22:58:47Z
dc.date.available2018-03-28T22:58:47Z
dc.identifierBrazilian Journal Of Medical And Biological Research. Assoc Bras Divulg Cientifica, v. 34, n. 4, n. 489, n. 492, 2001.
dc.identifier0100-879X
dc.identifierWOS:000168205400008
dc.identifier10.1590/S0100-879X2001000400008
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/78673
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/78673
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/78673
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1266804
dc.descriptionHereditary persistence, of fetal hemoglobin is an uncommon, benign disorder in which the expression of gamma -globin genes persists into adult life. Several point mutations have been associated with the increased gamma -globin gene promoter activity. We evaluated the -195 (C-->G) mutation by a functional in vitro assay based on the luciferase reporter gene system. The results indicated that the increased promoter activity observed in vivo could not be reproduced in vitro, under the conditions employed, suggesting that other factors may be involved in the overexpression of the gamma -globin gene containing the -195 (C-->G) mutation. Furthermore: this is the first time that the -195 (C-->G) mutation of the (A)gamma -globin gene has been evaluated by in vitro gene expression.
dc.description34
dc.description4
dc.description489
dc.description492
dc.languageen
dc.publisherAssoc Bras Divulg Cientifica
dc.publisherSao Paulo
dc.publisherBrasil
dc.relationBrazilian Journal Of Medical And Biological Research
dc.relationBrazilian J. Med. Biol. Res.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectfetal hemoglobin
dc.subjecthereditary persistence of fetal hemoglobin
dc.subjectHPFH
dc.subjecttransient expression
dc.subjectGamma-globin Gene
dc.subjectIncreases Promoter Strength
dc.subjectBrazilian Type
dc.subjectCell
dc.subjectHpfh
dc.subjectSubstitution
dc.subjectEnhancers
dc.subjectSequence
dc.titleThe (A)gamma-195 (C -> G) mutation in hereditary persistence of fetal hemoglobin is not associated with activation of a reporter gene in vitro
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución