Artículos de revistas
Analysis of the Contribution of Nonresident Progenitor Cells and Hematopoietic Cells to Reparative Dentinogenesis Using Parabiosis Model in Mice
Registro en:
Journal Of Endodontics. Elsevier Science Inc, v. 38, n. 9, n. 1214, n. 1219, 2012.
0099-2399
WOS:000308278200011
10.1016/j.joen.2012.05.016
Autor
Frozoni, M
Zaia, AA
Line, SRP
Mina, M
Institución
Resumen
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Introduction: The aim of this study was to analyze the contribution of nonresident progenitor/stem cells and hematopoietic cells to reparative dentinogenesis. Methods: Parabiosis was established between C57BL/6-TgN(ACTbEGFP)10sb/J transgenic mice (GFP+) and C57BL/6 wild-type mice (GFP-) to ensure blood cross-circulation between animals. Reparative dentinogenesis was stimulated by pulp exposures and capping on the first maxillary molar in the GFP- mice. Histologic sections of injured molars from GFP- Mice were analyzed by epifluorescence microscopy to examine the contributions of GFP+ cells (nonresident progenitor cells and hematopoietic cells originating from GFP+ mice) to reparative dentinogenesis. Results: GFP+ cells were detected in close association with reparative dentin formed at the site of pulp exposure in the maxillary first molars of the GFP- mice. Conclusions: The present study suggests the participation of the nonresident progenitor cells and hematopoietic cells in reparative dentinogenesis. (J Endod 2012;38:1214-1219) 38 9 1214 1219 American Association of Endodontists Foundation Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) NIH [DE016689] Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) CAPES [3422/09-7] NIH [DE016689]