Capture enzyme-linked immunosorbent assay to detect specific immunoglobulin E in sera of patients with paracoccidioidomycosis

dc.creatorMamoni, RL
dc.creatorRossi, CL
dc.creatorCamargo, ZP
dc.creatorBlotta, MHSL
dc.date2001
dc.dateSEP
dc.date2014-11-14T20:43:15Z
dc.date2015-11-26T16:08:24Z
dc.date2014-11-14T20:43:15Z
dc.date2015-11-26T16:08:24Z
dc.date.accessioned2018-03-28T22:56:57Z
dc.date.available2018-03-28T22:56:57Z
dc.identifierAmerican Journal Of Tropical Medicine And Hygiene. Amer Soc Trop Med & Hygiene, v. 65, n. 3, n. 237, n. 241, 2001.
dc.identifier0002-9637
dc.identifierWOS:000170933400015
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/63135
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/63135
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/63135
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1266350
dc.descriptionParacoccidioidomycosis (PCM) is the most frequent systemic mycosis in South America. The disease is characterized by a polyclonal activation of B cells, resulting in hyperimmunoglobulinemia. The production of immunoglobulin (Ig) E in deep mycosis has been related to the severity of the disease. However, the detection of specific IgE in sera of patients is difficult because of the competition with the IgG. We compared a capture and an indirect enzyme-linked immunosorbent assay (ELISA) technique to detect Paracoccidioides brasiliensis IgE. We found that the capture ELISA presented higher performance and lower background values than the indirect assay, resulting in a significant quantitative discrimination between sera from patients with the 2 major clinical forms of PCM. Patients with the juvenile form presented significantly higher levels of P. brasiliensis IgE, as compared with patients with the adult form. The capture ELISA was used in the follow-up of patients receiving treatment, showing that the levels of specific IgE decreased as the patient's clinical conditions improved.
dc.descriptiono TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE AGOSTO DE 2015.
dc.description65
dc.description3
dc.description237
dc.description241
dc.languageen
dc.publisherAmer Soc Trop Med & Hygiene
dc.publisherMclean
dc.publisherEUA
dc.relationAmerican Journal Of Tropical Medicine And Hygiene
dc.relationAm. J. Trop. Med. Hyg.
dc.rightsembargo
dc.sourceWeb of Science
dc.subjectGlycoprotein Gp43
dc.subjectInterferon-gamma
dc.subjectAntibody Capture
dc.subjectIge
dc.subjectBrasiliensis
dc.subjectMice
dc.subjectPulmonary
dc.subjectCytokines
dc.subjectBinding
dc.subjectQuantitation
dc.titleCapture enzyme-linked immunosorbent assay to detect specific immunoglobulin E in sera of patients with paracoccidioidomycosis
dc.typeArtículos de revistas


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