Artículos de revistas
Restriction Fragment Length Polymorphism of Urease C and Urease B Genes of Helicobacter pylori Strains Isolated from Brazilian Patients with Peptic Ulcer and Chronic Gastritis
Registro en:
Digestive Diseases And Sciences. Springer, v. 54, n. 7, n. 1487, n. 1493, 2009.
0163-2116
WOS:000266654900015
10.1007/s10620-008-0525-2
Autor
Roesler, BM
de Oliveira, TB
Bonon, SHA
Monici, LT
Zeitune, JMR
Costa, SCB
Institución
Resumen
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) The aim of the present work is to identify the presence of Helicobacter pylori bacterium in samples of gastric mucosa fragments, obtained by gastric biopsy, from Brazilian patients with peptic ulcer and chronic gastritis and also to determine differences among the prevalent strains in these two diseases by urease C and urease B genes amplification utilizing nested polymerase chain reaction (PCR) and PCR. We encountered 17 genotyping patterns for urease C and 7 for urease B and, although no significant differences were found among the patterns encountered for both diseases, we found predominant groups for each disease. Typing methods of the products obtained by nested PCR and PCR show a functional scheme and are of great importance for epidemiologic studies and H. pylori strain characterization, in addition to allowing correlation among the several strains and their role in the diseases caused by this microorganism. 54 7 1487 1493 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)