Endotoxin removal from solutions of F(ab ')(2) fragments of equine antibodies against snake venom using macroporous chitosan membrane

dc.creatorde Freitas, SS
dc.creatorMachado, RL
dc.creatorde Arruda, EJ
dc.creatorSantana, CC
dc.creatorBueno, SMA
dc.date2004
dc.dateMAY 1
dc.date2014-11-20T03:43:07Z
dc.date2015-11-26T16:04:41Z
dc.date2014-11-20T03:43:07Z
dc.date2015-11-26T16:04:41Z
dc.date.accessioned2018-03-28T22:53:48Z
dc.date.available2018-03-28T22:53:48Z
dc.identifierJournal Of Membrane Science. Elsevier Science Bv, v. 234, n. 41671, n. 67, n. 73, 2004.
dc.identifier0376-7388
dc.identifierWOS:000220884900007
dc.identifier10.1016/j.memsci.2003.12.019
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/64778
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/64778
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/64778
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1265548
dc.descriptionThe adsorptive filtration technique using chitosan membranes was used to remove endotoxin (ET) from buffers and bothrops antivenom serum (F(ab')(2) fragments of equine antibodies). Macroporous chitosan membrane was prepared from chitosan using silica particles as porogen agent. The effects of solution conditions on the efficiency of endotoxin removal and protein recovery were studied. The ET concentrations were reduced to 0.58 and 71.6 EU/ml, respectively, when Tris-HCl pH 7.0 buffer with 18.6 and 8092.5 EU/ml was passed through the chitosan membrane. This buffer was used to evaluate ET removal in the presence of the equine F(ab')(2) fragments. The results showed that the presence of protein in the feed solution did not influence much the clearance of this membrane. Removal of 97% of the ET and recovery of 94 + of the F(ab')2 were obtained at an initial ET and F(ab')(2) concentration of 974.4 EU/ml and 18.6 mg/ml, respectively. (C) 2004 Elsevier B.V. All rights reserved.
dc.description234
dc.description41671
dc.description67
dc.description73
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationJournal Of Membrane Science
dc.relationJ. Membr. Sci.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectdepyrogenation
dc.subjectendotoxins
dc.subjectsnake antivenom
dc.subjectchitosan
dc.subjectadsorptive membranes
dc.subjectProtein Solutions
dc.subjectSelective Removal
dc.subjectAdsorbers
dc.subjectChromatography
dc.subjectDepyrogenation
dc.subjectSeparation
dc.subjectHistidine
dc.titleEndotoxin removal from solutions of F(ab ')(2) fragments of equine antibodies against snake venom using macroporous chitosan membrane
dc.typeArtículos de revistas


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