Cross-neutralization Of The Neurotoxicity Of Crotalus Durissus Terrificus And Bothrops Jararacussu Venoms By Antisera Against Crotoxin And Phospholipase A2 From Crotalus Durissus Cascavella Venom

dc.creatorBeghini D.G.
dc.creatorDa Cruz-Hofling M.A.
dc.creatorRandazzo-Moura P.
dc.creatorRodrigues-Simioni L.
dc.creatorNovello J.C.
dc.creatorHyslop S.
dc.creatorMarangoni S.
dc.date2005
dc.date2015-06-26T14:06:35Z
dc.date2015-11-26T15:40:41Z
dc.date2015-06-26T14:06:35Z
dc.date2015-11-26T15:40:41Z
dc.date.accessioned2018-03-28T22:49:09Z
dc.date.available2018-03-28T22:49:09Z
dc.identifier
dc.identifierToxicon. , v. 46, n. 6, p. 604 - 611, 2005.
dc.identifier410101
dc.identifier10.1016/j.toxicon.2005.06.018
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-26444486968&partnerID=40&md5=9db50b24bc97382427bae517c3ad5fa1
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/93149
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/93149
dc.identifier2-s2.0-26444486968
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1264430
dc.descriptionWe have previously demonstrated that rabbit antisera raised against crotoxin from Crotalus durissus cascavella venom (cdc-crotoxin) and its PLA 2 (cdc-PLA2) neutralized the neurotoxicity of this venom and its crotoxin. In this study, we examined the ability of these antisera to neutralize the neurotoxicity of Crotalus durissus terrificus and Bothrops jararacussu venoms and their major toxins, cdt-crotoxin and bothropstoxin-I (BthTX-I), respectively, in mouse isolated phrenic nerve-diaphragm preparations. Immunoblotting showed that antiserum to cdc-crotoxin recognized cdt-crotoxin and BthTX-I, while antiserum to cdc-PLA2 recognized cdt-PLA 2 and BthTX-I. ELISA corroborated this cross-reactivity. Antiserum to cdc-crotoxin prevented the neuromuscular blockade caused by C. d. terrificus venom and its crotoxin at a venom/crotoxin:antiserum ratio of 1:3. Antiserum to cdc-PLA2 also neutralized the neuromuscular blockade caused by C. d. terrificus venom or its crotoxin at venom or toxin:antiserum ratios of 1:3 and 1:1, respectively. The neuromuscular blockade caused by B. jararacussu venom and BthTX-I was also neutralized by the antisera to cdc-crotoxin and cdc-PLA 2 at a venom/toxin:antiserum ratio of 1:10 for both. Commercial equine antivenom raised against C. d. terrificus venom was effective in preventing the neuromuscular blockade typical of B. jararacussu venom (venom:antivenom ratio of 1:2), whereas for BthTX-I the ratio was 1:10. These results show that antiserum produced against PLA2, the major toxin in C. durissus cascavella venom, efficiently neutralized the neurotoxicity of C. d. terrificus and B. jararacussu venoms and their PLA2 toxins.
dc.description46
dc.description6
dc.description604
dc.description611
dc.descriptionBeghini, D.G., Toyama, M.H., Hyslop, S., Sodek, L., Novello, J.C., Marangoni, S., Enzymatic characterization of a novel phospholipase A2 from crotalus durissus cascavella rattlesnake (maracambóia) venom (2000) J. Protein Chem., 19, pp. 603-607
dc.descriptionBeghini, D.G., Hernandez-Oliveira, S., Rodrigues-Simioni, L., Novello, J.C., Hyslop, S., Marangoni, S., Anti-sera raised in rabbits against crotoxin and phospholipase A 2 from Crotalus durissus cascavella venom neutralize the neurotoxicity of the venom and crotoxin (2004) Toxicon, 44, pp. 141-148
dc.descriptionBeghini, D.G., Rodrigues-Simioni, L., Toyama, M.H., Novello, J.C., Cruz-Höfling, M.A., Marangoni, S., Neurotoxic and myotoxic actions of crotoxin 'like' and crotalus durissus cascavella whole venom in the chick biventer cervicis preparation (2004) Toxicon, 43, pp. 255-261
dc.descriptionBrazil, V., Contribuição ao estudo do veneno ophidico. III. Tratamento das mordeduras de cobras (1903) Rev. Med. São Paulo, 6, pp. 265-278
dc.descriptionBrazil, V., Pestana, R., Nova contribuicao ao estudo do envenenamento ophidico (1909) Ver. Méd. São Paulo, 19, pp. 21-22
dc.descriptionBreithaupt, H., Enzymatic characteristics of Crotalus phospholipase A2 and the crotoxin complex (1976) Toxicon, 14, pp. 221-233
dc.descriptionBülbring, E., Observation on the isolated phrenic nerve diaphragm preparation of the ratr (1946) Br. J. Pharmacol., 1, pp. 38-61
dc.descriptionChavez-Olórtegui, C., Silva Lopes, C., Drumond Cordeiro, F., Granier, C., Diniz, C.R., An enzyme linked immunosorbent assay (ELISA) that discriminates between Bothrops atrox and Lachesis muta muta venoms (1993) Toxicon, 31, pp. 417-425
dc.descriptionChoumet, V., Jiang, M.S., Radvanyi, F., Ownby, C., Bon, C., Neutralization of lethal potency and inhibition of enzymatic activity of a phospholipase A2 neurotoxin, crotoxin, by non-precipitating antibodies (Fab) (1989) Fed. Eur. Biochem. Soc. Lett., 244, pp. 167-173
dc.descriptionChoumet, V., Faure, G., Robbe-Vincent, A., Saliou, B., Mazié, J.C., Bon, C., Immunochemical analysis of a snake venom phospholipase A2 neurotoxin, crotoxin, with monoclonal antibodies (1992) Mol. Immunol., 29, pp. 871-882
dc.descriptionCintra, A.C., Marangoni, S., Oliveira, B., Giglio, J.R., Bothropstoxin-I: Amino acid sequence and function (1993) J. Protein Chem., 12, pp. 57-64
dc.descriptionDennis, E.A., Diversity of group types, regulation, and function of phospholipase A2 (1994) J. Biol. Chem., 269, pp. 13057-13060
dc.descriptionDessen, A., Phospholipase A2 enzymes: Structural diversity in lipid messenger metabolism (2000) Struct. Fold Des., 8, pp. 15-R22
dc.descriptionDos-Santos, M.C., Gonçalvez, L.R., Fortes-Dias, C.L., Cury, Y., Gutiérrez, J.M., Furtado, M.F., Estudo comparativo do efeito neutralizante dos soros antibotrópico (SAB) e antibotrópico/crotálico (SAB-C) sobre atividade miotóxica do veneno de Bothrops jararacussu (Bjssu) em camundongos (1990) Mem. Inst. Butantan, 52, p. 71
dc.descriptionDos-Santos, M.C., Gonçalvez, L.R., Fortes-Dias, C.L., Cury, Y., Gutiérrez, J.M., Furtado, M.F., A eficácia do antiveneno botrópico-crotálico na neutralização das principais atividades do veneno de Bothrops jararacussu (1992) Rev. Inst. Med. Trop. S. Paulo, 34, pp. 77-83
dc.descriptionFreitas, T.V., Fortes-Dias, C.L., Diniz, C.R., Protection against the lethal effects of Crotalus durissus terrificus (South American rattlesnake) venom in animals immunized with crotoxin (1990) Toxicon, 28, pp. 1491-1496
dc.descriptionFuly, A.L., Soares, A.M., Marcussi, S., Giglio, J.R., Guimarães, J.A., Signal transduction pathways involved in the platelet aggregation induce by a D-49 phospholipase isolated from Bothrops jararacussu snake venom (2004) Biochimie, 86, pp. 731-739
dc.descriptionHeluany, N.F., Homsi-Brandeburgo, M.I., Giglio, J.R., Prado-Franceschi, J., Rodrigues-Simioni, L., Effects induced by bothropstoxin, a component from bothrops jararacussu snake venom on mouse and chick muscle preparations (1992) Toxicon, 30, pp. 1203-1210
dc.descriptionHendon, R.A., Fraenkel-Conrat, H., Biological roles of the two components of crotoxin (1971) Proc. Natl Acad. Sci. USA, 68, pp. 1560-1563
dc.descriptionHomsi-Brandeburgo, M.I., Queiroz, L.S., Santo-Neto, H., Rodrigues-Simioni, L., Giglio, J.R., Fractionation of bothrops jararacussu snake venom: Partial chemical characterization and biological activity of bothropstoxin (1988) Toxicon, 26, pp. 615-627
dc.descriptionKashima, S., Roberto, P.G., Soares, A.M., Asolfi-Filho, S., Pereira, J.O., Giuliati, S., Faria Jr., M., França, S.C., Analysis of Bothrops jararacussu venomous gland transcriptome focusing on structural and functional aspects: I. Gene expression profile of highly expressed phospholipases A2 (2004) Biochimie, 86, pp. 211-219
dc.descriptionKetelhut, D.F.J., Homem De Mello, M., Veronese, E.L.G., Esmeraldino, L.E., Murakami, M.T., Arni, R.K., Giglio, J.R., Sampaio, S.V., Isolation, characterization and biological activity of acidic phospholipase A2 isoforms from Bothrops jararacussu snake venom (2003) Biochimie, 85, pp. 983-991
dc.descriptionKini, R.M., Excitement ahead: Structure, function and mechanism of snake venom phospholipase A2 enzymes (2003) Toxicon, 42, pp. 827-840
dc.descriptionLanducci, E.C., Condino-Neto, A., Perez, A.C., Hyslop, S., Corrado, A.P., Novello, J.C., Marangoni, S., De Nucci, G., Crotoxin induces aggregation of human washed platelets (1994) Toxicon, 32, pp. 217-226
dc.descriptionMelgarejo, A.R., Serpentes peçonhentas do Brasil (2003) Animais Peçonhentos no Brasil Biologia, Clínica e Terapêutica Dos Acidentes, pp. 33-61. , J.L.C. Cardoso F.O. França F. Hui Wen C.M. Málaque V. Haddad Jr Sarvier São Paulo
dc.descriptionMénez, A., Molecular immunology of snake toxins (1985) Pharmacol. Ther., 30, pp. 91-113
dc.descriptionMiddlebrook, J.L., Kaiser, S., Immunological relationship of phospholipase A2 neurotoxins from snake venoms (1989) Toxicon, 22, pp. 965-977
dc.descriptionMollier, P., Chwetzoff, S., Menez, A., A monoclonal antibody recognizing a conserved epitope in a group of phospholipases A2 (1990) Mol. Immunol., 27, pp. 7-15
dc.descriptionOshima-Franco, Y., Hyslop, S., Prado-Franceschi, J., Cruz-Höfling, M.A., Rodrigues-Simioni, L., Neutralizing capacity of antisera raised in horses and rabbits against crotalus durissus terrificus (South American rattlesnake) venom and its main toxin, crotoxin (1999) Toxicon, 37, pp. 1341-1457
dc.descriptionOshima-Franco, Y., Leite, G.B., Silva, G.H., Cardoso, D.F., Hyslop, S., Giglio, J.R., Cruz-Höfling, M.A., Rodrigues-Simioni, L., Neutralization of the pharmacological effects of bothropstoxin-I from Bothrops jararacussu (jararacuçu) venom by crotoxin antiserum and heparin (2001) Toxicon, 39, pp. 1477-1485
dc.descriptionPereira, M.F., Novello, J.C., Cintra, A.C., Giglio, J.R., Landucci, E.T., Oliveira, B., Marangoni, S., The amino acid sequence of bothropstoxin-II, an Asp-49 myotoxin from Bothrops jararacussu (jararacuçu) venom with low phospholipase A 2 activity (1998) J. Protein Chem., 17, pp. 381-386
dc.descriptionSantoro, M.L., Sousa-E-Silva, M.C.C., Gonçalvez, L.R.C., Almeida-Santos, S.M., Cardoso, D.F., Laporta-Ferreira, I.L., Saiki, M., Sano-Martins, I.S., Comparison of the biological activities in venoms from three subspecies of the South American rattlesnake (Crotalus durissus terrificus, C. durissus cascavella and C. durissus collilineatus) (1999) Comp. Biochem. Physiol., 122, pp. 61-73
dc.descriptionSlotta, K.H., Fraenkel-Conrat, H.L., Schlangengifte. III: Mitteilung reiningung und Krystallization des klapperschangengiftes (1938) Ber. Dich. Chem. Ges., 71, pp. 1076-1081
dc.descriptionStrong, P.N., Wood, J.N., Ivanyi, J., Characterization of monoclonal antibodies against β-BTX and their use as structural probes for related phospholipase A2 enzymes and presynaptic phospholipase neurotoxins (1984) Eur. J. Biochem., 142, pp. 145-151
dc.descriptionVital Brazil, O., Pharmacology of crystalline crotoxin. II. Neuromuscular blocking action (1966) Mem. Inst. Butantan, 33, pp. 981-992
dc.descriptionZamuner, S.R., Da Cruz-Hofling, M.A., Corrado, A.P., Hyslop, S., Rodrigues-Simioni, L., Comparison of the neurotoxic and myotoxic effects of brazilian bothrops venoms and their neutralization by commercial antivenom (2004) Toxicon, 44, pp. 259-271
dc.languageen
dc.publisher
dc.relationToxicon
dc.rightsfechado
dc.sourceScopus
dc.titleCross-neutralization Of The Neurotoxicity Of Crotalus Durissus Terrificus And Bothrops Jararacussu Venoms By Antisera Against Crotoxin And Phospholipase A2 From Crotalus Durissus Cascavella Venom
dc.typeArtículos de revistas


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