Artículos de revistas
Nitrite Reduction And Superoxide-dependent Nitric Oxide Degradation By Arabidopsis Mitochondria: Influence Of External Nad(p)h Dehydrogenases And Alternative Oxidase In The Control Of Nitric Oxide Levels
Registro en:
Nitric Oxide - Biology And Chemistry. , v. 21, n. 2, p. 132 - 139, 2009.
10898603
10.1016/j.niox.2009.06.003
2-s2.0-68149162554
Autor
Wulff A.
Oliveira H.C.
Saviani E.E.
Salgado I.
Institución
Resumen
Mitochondria recently have emerged as important sites in controlling NO levels within the cell. In this study, the synthesis of nitric oxide (NO) from nitrite and its degradation by mitochondria isolated from Arabidopsis thaliana were examined. Oxygen and NO concentrations in the reaction medium were measured with specific electrodes. Nitrite inhibited the respiration of isolated A. thaliana mitochondria, in competition with oxygen, an effect that was abolished or potentiated when electron flow occurred via alternative oxidase (AOX) or cytochrome c oxidase (COX), respectively. The production of NO from nitrite was detected electrochemically only under anaerobiosis because of a superoxide-dependent process of NO degradation. Electron leakage from external NAD(P)H dehydrogenases contributed the most to NO degradation as higher rates of Amplex Red-detected H2O2 production and NO consumption were observed in NAD(P)H-energized mitochondria. 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