Artículos de revistas
Rapid Method For The Determination Of Organic Acids In Wine By Capillary Electrophoresis With Indirect Uv Detection
Registro en:
Food Control. , v. 20, n. 6, p. 548 - 552, 2009.
9567135
10.1016/j.foodcont.2008.08.004
2-s2.0-60449111942
Autor
Peres R.G.
Moraes E.P.
Micke G.A.
Tonin F.G.
Tavares M.F.M.
Rodriguez-Amaya D.B.
Institución
Resumen
A capillary electrophoresis method for organic acids in wine was developed and validated. The optimal electrolyte consisted of 10 mmol/L 3,5-dinitrobenzoic acid (DNB) at pH 3.6 containing 0.2 mmol/L cetyltrimethylammonium bromide as flow reverser. DNB was chosen because it has an effective mobility similar to the organic acids under investigation, good buffering capacity at pH 3.6, and good chromophoric characteristics for indirect UV-absorbance detection at 254 nm. Sample preparation involved dilution and filtration. The method showed good performance characteristics: Linearity at 6 to 285 mg/L (r > 0.99); detection and quantification limits of 0.64 to 1.55 and 2.12 to 5.15 mg/L, respectively; separation time of less than 5.5 min. Coefficients of variation for ten injections were less than 5% and recoveries varied from 95% to 102%. 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