Artículos de revistas
Microbial Load Of Reprocessable Trocars After Gynecological Videolaparoscopy [carga Microbiana De Trocartes Reprocessáveis Após Laparoscopias Ginecológicas]
Registro en:
Revista Brasileira De Ginecologia E Obstetricia. , v. 31, n. 12, p. 586 - 591, 2009.
1007203
2-s2.0-75649144720
Autor
Vilas-Boas V.A.
Levy C.E.
de Freitas M.I.P.
Institución
Resumen
Purpose: to identify the microbial charge present in reusable trocars used in gynecological laparoscopies. Methods: a descriptive exploratory study. An amount of 57 trocars, 30 with 10 mm of diameter and 27 with 5 mm, have been collected from the surgical unit, immediately after the surgery and placed in a sterilized recipient, in which 250 mL of sterile distilled water was added. Then, the trocars were agitated for the drainage of particles and to obtain a wash-out fluid to be analyzed. After being filtered through 0.22 μm cellulose membrane, the residue was placed on blood agar plates with a sterilized forceps. Following incubation, microbiological analysis has been done to count the number of colonies and further identify the microorganisms, using standard laboratorial techniques. Results: microbial charge was recovered from 47.4% of the trocars analyzed. Among those, 45.6% presented 1 to 100 growing colonies. Fourteen types of microorganisms have been identified, among which the more frequently isolated were coagulase- negative Staphylococcus (28%) and Bacillus sp (21%), Aeromonas hydrophila, Alcaligenes sp, Candida parapsilosis, and enterobacteries were also identified. Conclusions: the study has demonstrated that the microbial challenge faced by the technician responsible for the cleaning and sterilization of trocars is low, as compared to the challenge imposed by biological markers. Nevertheless, it may be not inferred that the risks for infectious complications for patients are minimal. 31 12 586 591 (2009) Dispõe Sobre As Medidas Para Redução Da Ocorrência De Infecções Por Micobactérias De Crescimento Rápido - Mcr Em Serviços De Saúde, 1 (40), p. 62. , Brasil. Agência Nacional de Vigilância Sanitária. Resolução RDC n. 8, 27 de fevereiro, Diário Oficial da União, Brasília: 2009;Seç Rutala, W.A., Weber, D.J., (2008) Healthcare Infection Control Practices Advisory Committee, , Guideline for disinfection and sterilization in healthcare facilities, 2008. Atlanta: CDC Chan-Myers, H., McAlister, D., Antonoplos, P., Natural bioburden levels detected on rigid lumened medical devices before and after cleaning (1997) Am J Infect Control, 25 (6), pp. 471-476 Chu, N.S., McAlister, D., Antonoplos, P.A., Natural bioburden levels detected on flexible gastrointestinal endoscopes after clinical use and manual cleaning (1998) Gastrointest Endosc, 48 (2), pp. 137-142 Pinto, F.M.G., Souza, R.Q., Silva, C.B., Mimica, L.M.J., Graziano, K.U., Analysis of the microbial load in the instruments used in orthopaedic surgeries (2009) Am J Infect Control, , In press Chu, N.S., Chan-Myers, H., Ghazanfari, N., Antonoplos, P., Levels of naturally occurring microorganisms on surgical instruments after clinical use and after washing (1999) Am J Infect Control, 27 (4), pp. 315-319 Rutala, W.A., Gergen, M.F., Jones, J.F., Weber, D.J., Levels of microbial contamination on surgical instruments (1998) Am J Infect Control, 26 (2), pp. 143-145 Nyström, B., Disinfection of surgical instruments (1981) J Hosp Infect, 2 (4), pp. 363-368 Eigner, U., Schmid, A., Wild, U., Bertsch, D., Fahr, A.M., Analysis of the comparative workflow and performance characteristics of the VITEK 2 and Phoenix systems (2005) J Clin Microbiol, 43 (8), pp. 3829-3834 Neri, V., Fersini, A., Ambrosi, A., Tartaglia, N., Valentino, T.P., Umbilical port-site complications in laparoscopic cholecystectomy: Role of topical antibiotic therapy (2008) Jsls, 12 (2), pp. 126-132 Colombo, A.L., Guimarães, T., Epidemiologia das infecções hematogênicas por Candida spp (2003) Rev Soc Bras Med Trop, 36 (5), pp. 599-607 Andreollo, N.A., Coelho Neto, J.S., Lopes, L.R., Brandalise, N.A., Leonardi, L.S., A laparoscopia no diagnóstico das doenças intra-abdominais: Análise de 168 casos (1999) Rev Assoc Med Bras, 45 (1), pp. 34-38