Drosophila Mediopunctata Microsatellites I: More Than A Hundred Polymorphic Loci Available For Genetic Studies

dc.creatorLaborda P.R.
dc.creatorMori G.M.
dc.creatorde Souza A.P.
dc.date2009
dc.date2015-06-26T13:33:26Z
dc.date2015-11-26T15:32:35Z
dc.date2015-06-26T13:33:26Z
dc.date2015-11-26T15:32:35Z
dc.date.accessioned2018-03-28T22:41:03Z
dc.date.available2018-03-28T22:41:03Z
dc.identifier
dc.identifierConservation Genetics Resources. , v. 1, n. 1, p. 297 - 307, 2009.
dc.identifier18777252
dc.identifier10.1007/s12686-009-9070-3
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84855961528&partnerID=40&md5=bbebb4045f2241337906fdd0b9e79fbd
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/91713
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/91713
dc.identifier2-s2.0-84855961528
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1262516
dc.descriptionWe describe the first 134 polymorphic microsatellites loci developed for the fly Drosophila mediopunctata. A genomic DNA library double-enriched for dinucleotides was constructed and approximately 600 unambiguous microsatellites were identified. The DNA library consisted of 60% positive clones, short-sized dinucleotides (mean = 8.7 repeats) and 73% of AC/GT combinations. A total of 177 primer pairs were designed and thirteen strains were used to achieve optimal PCR amplification conditions and allelic variability of the isolated loci. The average number of alleles per locus was 4.6 and means of observed and expected heterozygosities were 0.23 and 0.62, respectively. The discriminating power average was 0.72. These new developed markers will be applied in the study of natural population dynamics and for the construction of a molecular linkage map for the species. © Springer Science+Business Media B.V. 2009.
dc.description1
dc.description1
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dc.description307
dc.descriptionAnanina, G., Peixoto, A.A., Souza, W.N., da Silva, L.B., Klaczko, L.B., Polytene chromosome map and inversion polymorphism in Drosophila mediopunctata (2002) Mem Inst Oswaldo Cruz, 97, pp. 691-694
dc.descriptionAnanina, G., Peixoto, A.A., Bitner-Mathé, B.C., Souza, W.N., da Silva, L.B., Valente, V.L.S., Klaczko, L.B., Chromosomal inversion polymorphism in Drosophila mediopunctata: Seasonal, altitudinal, and latitudinal variation (2004) Genet Mol Biol, 27, pp. 61-69
dc.descriptionAndrade, C.A.C., Hatadani, L.M., Klaczko, L.B., Phenotypic plasticity of the aedeagus of Drosophila mediopunctata: Effect of the temperature (2005) J Therm Biol, 30, pp. 518-523
dc.descriptionAndrade, C.A.C., Vieira, R.D., Ananina, G., Klaczko, L.B., Evolution of the male genitalia: Morphological variation of the aedeagi in a natural population of Drosophila mediopunctata (2009) Genetica, 135, pp. 13-23
dc.descriptionAzeredo-Espin, A.M.L., Schroder, R.F.W., Huettel, M.D., Sheppard, W.S., Mitochondiral-DNA variation in geographic populations of Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera, Chrisomelidae) (1991) Experientia, 47, pp. 483-485
dc.descriptionBillotte, N., Lagoda, P.J.L., Risterucci, A.-M., Baurens, F.-C., Microsatellite- enriched libraries: Applied methodology for the development of SSR markers in tropical crops (1999) Fruits, 54, pp. 277-288
dc.descriptionBitner-Mathé, B.C., Peixoto, A.A., Klaczko, L.B., Morphological variation in a natural population of Drosophila mediopunctata: Altitudinal cline, temporal changes and influence of chromosome inversions (1995) Heredity, 75, pp. 54-61
dc.descriptionCarvalho, A.B., Klaczko, L.B., Autosomal suppressors of sex-ratio in Drosophila mediopunctata (1993) Heredity, 71, pp. 546-551
dc.descriptionCarvalho, A.B., Peixoto, A.A., Klaczko, L.B., Sex-ratio in Drosophila mediopunctata (1989) Heredity, 62, pp. 425-428
dc.descriptionCreste, S., Tulmann Neto, A., Figueira, A., Detection of single sequence repeat polymorphisms in denaturing polyacrylamide sequencing gels by silver staining (2001) Plant Mol Biol Rep, 19, pp. 299-306
dc.descriptionDobzhansky, T., Pavan, C., (1943) Studies on Brazilian Species of Drosophila, , Bol Facul Fil Cien Let Univ S Paulo n36 Biologia Geral n4
dc.descriptionHatadani, L.M., Klaczko, L.B., Shape and size variation on the wing of Drosophila mediopunctata: Influence of chromosome inversions and genotype-environment interaction (2008) Genetica, 133, pp. 335-342
dc.descriptionIhaka, R., Gentleman, R., R: A language for data analysis and graphics (1996) J Comput Graph Stat, 5, pp. 299-314
dc.descriptionKatti, M.V., Rankejar, P.K., Gupta, V.S., Differential distribution of simple sequence repeats of eukaryotic genome sequences (2001) Mol Biol Evol, 18, pp. 1161-1167
dc.descriptionMiller, M.P., (1997) Tools for Population Genetic Analyses (TFPGA) 1.3: A Windows Program for the Analysis of Allozyme and Molecular Population Genetic Data
dc.descriptionNei, M., Estimation of average heterozygosity and genetic distance from a small number of individuals (1978) Genetics, 89, pp. 583-590
dc.descriptionRaymond, M., Rousset, F., GENEPOP (version 1.2): Population genetics software for exact tests and ecumenicism (1995) J Hered, 86, pp. 248-249
dc.descriptionRocha, F., Medeiros, H.F., Klaczko, L.B., The reaction norm for abdominal pigmentation and its curve in Drosophila mediopunctata depend on the mean phenotypic value (2008) Evolution, 63, pp. 280-287
dc.descriptionRozen, S., Skaletsky, H.J., Primer3 on the WWW for general users and for biologist programmers (2000) Bioinformatics Methods and Protocols: Methods in Molecular Biology, pp. 365-386. , In: Krawetz S, Misener S (eds), Humana Press, Totowa
dc.descriptionSchug, M.D., Wtterstrand, K.A., Gaudette, M.S., Lim, R.H., Hutter, C.M., Aquadro, C.F., The distribution and frequency of microsatellite loci in Drosophila melanogaster (1998) Mol Ecol, 7, pp. 57-70
dc.descriptionTemnykh, S., Declerck, G., Lukashova, A., Lipovich, L., Cartinhour, S., McCouch, S., Computational and experimental analysis of microsatellites in rice (Oryza sativa L.): Frequency, length variation, transposon associations, and genetic marker potential (2001) Genome Res, 11, pp. 1441-1452
dc.descriptionTessier, C., David, J., This, P., Boursiquot, J.M., Charrier, A., Optimization of the choice of molecular markers for varietal identification in Vitis vinifera L (1999) Theor Appl Genet, 98, pp. 171-177
dc.descriptionTóth, G., Gáspari, Z., Jurka, J., Microsatellites in different eukaryotic genomes: Survey and analysis (2000) Genome Res, 10, pp. 967-981
dc.descriptionVilela, C.R., On the Drosophila tripunctata species group (Diptera, Drosophilidae) (1992) Rev Bras Entomol, 36, pp. 197-221
dc.languageen
dc.publisher
dc.relationConservation Genetics Resources
dc.rightsfechado
dc.sourceScopus
dc.titleDrosophila Mediopunctata Microsatellites I: More Than A Hundred Polymorphic Loci Available For Genetic Studies
dc.typeArtículos de revistas


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