Artículos de revistas
Genetic Polymorphism Of Streptococcus Mutans Strains Associated With Incomplete Caries Removal
Registro en:
Brazilian Journal Of Oral Sciences. , v. 8, n. 1, p. 2 - 8, 2009.
16773217
2-s2.0-75949130217
Autor
Duque C.
de Cassia Negrini T.
Sacono N.T.
Gomes Boriollo M.F.
Hofling J.F.
Hebling J.
Palomari Spolidorio D.M.
Institución
Resumen
Aim: Despite the antibacterial properties of dental materials, the survival of residual bacteria under restorations has been demonstrated after incomplete caries removal. The aim of this study was to evaluate the genetic polymorphism of Streptococcus mutans strains isolated from deep dentinal lesions before and three months after incomplete caries removal. Methods: Samples of carious dentin were collected from 33 primary and/or permanent molars before and after indirect pulp treatment and processed for microbiological isolation of mutans streptococci (MS). After three months of the dental treatment, positive cultures for MS were detected in only ten of these teeth. DNA of MS isolates were obtained and subjected to polymerase chain reaction (PCR) for identification of S mutans. The arbitrary primed-PCR method (primer OPA-13) was used to detect the genetic polymorphism of S. mutans strains. Results: Identical or highly related S. mutans genotypes were observed in each tooth, regardless of the collect. Considering each tooth separately, a maximum of nine genotypic patterns were found in each tooth from all the collects. In addition, at least one genotypic pattern was repeated in the three collects. Genetic diversity was observed among the S. mutans isolates, obtained from different teeth after three months of the dental treatment. Conclusions: The persistence of identical genotypic patterns and the genetic similarity among the isolates, from the same tooth in distinct collects, showed the resistance of some S. mutans strains after incomplete caries removal treatment. 8 1 2 8 Bowden, G.H.W., Hamilton, I.R., Survival of oral bacteria (1998) Crit Rev Oral Biol Med, 9, pp. 54-85 Cvitkovitch, D.G., Genetic competence and transformation in oral streptococci (2001) Crit Rev Oral Biol Med, 12, pp. 217-243 Fejerskov, O., Concepts of dental caries and their consequences for understanding the disease (1997) Community Dent Oral Epidemiol, 25, pp. 5-12 Coykendall, A.L., Classification and identification of the viridans streptococci (1989) Clin Microbiol Rev, 2, pp. 315-328 Loesche, W.J., Role of Streptococcus mutans in dental decay (1986) Microbiol Rev, 50, pp. 353-380 Quivey, R.G., Kuhnert, W.L., Hahn, K., Genetics of acid adaptation in oral streptococci (2001) Crit Rev Oral Biol Med, 12, pp. 301-314 Homer, K.A., Patel, R., Beighton, D., Effects of N-acetylglucosamine on carbohydrate fermentation by Streptococcus mutans NT 10449 and Streptococcus sobrinus SL-1 (1993) Infect Immun, 61, pp. 295-302 Abranches, J., Chen, Y.Y., Burne, R.A., Galactose metabolism by Streptococcus mutans (2004) Appl Environ Microbiol, 70, pp. 6047-6052 Arthur, R.A., Tabchoury, C.P., Mattos-Graner, R.O., Del Bel Cury, A.A., Paes Leme, A.F., Vale, G.C., Genotypic diversity of S. mutans in dental biofilm formed in situ under sugar stress exposure (2007) Braz Dent J, 18, pp. 185-191 Saarela, M., Hannula, J., Mättö, J., Asikainen, S., Alaluusua, S., Typing of mutans streptococci by arbitrarily primed polymerase chain reaction (1996) Arch Oral Biol, 41, pp. 821-826 Redmo Emanuelsson, I., Wang, X.M., Demonstration of identical strains of mutans streptococci within Chinese families by genotyping (1998) Eur J Oral Sci, 106, pp. 788-794 Grönroos, L., Alaluusua, S., Site-specific oral colonization of mutans streptococci detected by arbitrarily primed PCR fingerprinting (2000) Caries Res, 34, pp. 474-480 Spolidorio, D.M.P., Höfling, J.F., Pizzolitto, A.C., Rosa, E.A., Negrini, T.C., Spolidorio, L.C., Genetic polymorphism of Streptococcus mutans in Brazilian family members (2003) Braz J Microbiol, 34, pp. 213-217 Napimoga, M.H., Kamiya, R.U., Rosa, R.T., Rosa, E.A., Höfling, J.F., Mattos-Graner, R.O., Genotypic diversity and virulence traits of Streptococcus mutans in caries-free and caries-active individuals (2004) J Med Microbiol, 53, pp. 697-703 Paddick, J.S., Brailsford, S.R., Kidd, E.A., Beighton, D., Phenotypic and genotypic selection of microbiota surviving under dental restorations (2005) Appl Environ Microbiol, 71, pp. 2467-2472 Truong, T.L., Ménard, C., Mouton, C., Trahan, L., Identification of mutans and other oral streptococci by random amplified polymorphic DNA analysis (2000) J Med Microbiol, 49, pp. 63-71 Alaluusua, S., Mättö, J., Grönroos, L., Innilä, S., Torkko, H., Asikainen, S., Oral colonization by more than one clonal type of mutans streptococcus in children with nursing-bottle dental caries (1996) Arch Oral Biol, 41, pp. 167-173 Nascimento, M.M., Höfling, J.F., Gonçalves, R.B., Streptococcus mutans genotypes isolated from root and coronal caries (2004) Caries Res, 38, pp. 454-463 Bjorndal, L., Larsen, T., Thylstrup, A., A clinical and microbiological study of deep carious lesions during stepwise excavation using long treatment intervals (1997) Caries Res, 31, pp. 411-417 Kreulen, C.M., de Soet, J.J., Weerheijm, K.L., van Amerongen, W.E., In vivo cariostatic effect of resin modified glass ionomer and amalgam on dentine (1997) Caries Res, 31, pp. 384-389 Weerheijm, K.L., Kreulen, C.M., de Soet, J.J., Groen, H.J., van Amerongen, W.E., Bacterial counts in carious dentine under restorations: 2-years in vivo effects (1999) Caries Res, 33, pp. 130-134 Davey, A.L., Rogers, A.H., Multiple types of the bacterium Streptococcus mutans in the human mouth and their intra-family transmission (1984) Arch Oral Biol, 29, pp. 453-460 Nociti F.H., Jr., Cesco de Toledo, R., Machado, M.A., Stefani, C.M., Line, S.R., Gonçalves, R.B., Clinical and microbiological evaluation of ligature-induced peri-implantitis and periodontitis in dogs (2001) Clin Oral Implants Res, 12, pp. 295-300 Oho, T., Yamashita, Y., Shimazaki, Y., Kushiyama, M., Koga, T., Simple and rapid detection of Streptococcus mutans and Streptococcus sobrinus in human saliva by polymerase chain reaction (2000) Oral Microbiol Immunol, 15, pp. 258-262 Castillo, A., Rubiano, S., Gutiérrez, J., Hermoso, A., Liébana, J., Post-pH effect in oral streptococci (2000) Clin Microbiol Infect, 6, pp. 142-146 Duque, C., Negrini, T.deC., Hebling, J., Spolidorio, D.M., Inhibitory activity of glass-ionomer cements on cariogenic bacteria (2005) Oper Dent., 30, pp. 636-640 Robinson, W.G., Old, L.A., Shah, D.S.H., Russell, R.R., Chromosomal insertions and deletions in Streptococcus mutans (2003) Caries Res, 37, pp. 148-156 Hohwy, J., Reinholdt, J., Kilian, M., Population dynamics of Streptococcus mitis in its natural habitat (2001) Infect Immun, 69, pp. 6055-6063 Dowson, C.G., Barcus, V., King, S., Pickerill, P., Whatmore, A., Yeo, M., Horizontal gene transfer and the evolution of resistance and virulence determinants in Streptococcus (1997) Soc Appl Bacteriol Symp Ser, 26, pp. 42S-51S Perry, D., Wondrack, L.M., Kuramitsu, H.K., Genetic transformation of putative cariogenic properties in Streptococcus mutans (1983) Infect Immun, 41, pp. 722-727 Chansley, P.E., Kral, T.A., Transformation of fluoride resistance genes in Streptococcus mutans (1989) Infect Immun, 57, pp. 1968-1970 Murchison, H.H., Barrett, J.F., Cardineau, G.A., Curtiss, R., Transformation of Streptococcus mutans with chromosomal and shuttle plasmid (pYA629) DNAs (1986) Infect Immun, 54, pp. 273-282 Kuramitsu, H.K., Trapa, V., Genetic exchange between oral streptococci during mixed growth (1984) J Gen Microbiol, 130, pp. 2497-2500 Li, Y.H., Lau, P.C., Lee, J.H., Ellen, R.P., Cvitkovitch, D.G., Natural genetic transformation of Streptococcus mutans growing in biofilms (2001) J Bacteriol, 183, pp. 897-908 Klein, M.I., Flório, F.M., Pereira, A.C., Höfling, J.F., Gonçalves, R.B., Longitudinal study of transmission, diversity and stability of Streptococcus mutans and Streptococcus sobrinus genotypes in Brazilian nursery children (2004) J Clin Microbiol, 42, pp. 4620-4626 Lindquist, B., Emilson, C.G., Colonization of Streptococcus mutans and Streptococcus sobrinus genotypes and caries development in children to mothers harboring both species (2004) Caries Res, 38, pp. 95-103 Mattos-Graner, R.O., Li, Y., Caufield, P.W., Duncan, M., Smith, D.J., Genotypic diversity of mutans streptococci in Brazilian nursery children suggests horizontal transmission (2001) J Clin Microbiol, 39, pp. 2313-2316