Artículos de revistas
Isolation And Preliminary Enzymatic Characterization Of A Novel Pla2 From Crotalus Durissus Collilineatus Venom
Registro en:
Journal Of Protein Chemistry. , v. 21, n. 3, p. 131 - 136, 2002.
2778033
10.1023/A:1015332314389
2-s2.0-0036241065
Autor
Ponce-Soto L.A.
Toyama M.H.
Hyslop S.
Novello J.C.
Marangoni S.
Institución
Resumen
A crotoxin homolog was purified from the Crotalus durissus collilineatus venom using molecular exclusion and reverse-phase HPLC. This crotoxin contained one PLA2 (Cdcolli III F6) and four crotapotin isoforms, whereas crotoxin from Crotalus durissus terrificus venom had three PLA2 isoforms and two crotapotin isoforms. SDS-PAGE showed that the C. d. collilineatus PLA2 and crotapotin had relative molecular mass of 15 and 9 kDa, respectively. Neither the PLA2 (Cdcolli III F6) nor the crotapotins (Cdcolli III F3 and F4) had any neurotoxicity in mouse phrenic nervediaphragm preparations when tested alone. However, when PLA2 and crotapotin were coincubated before testing, the neurotoxicity was restored to a level similar to test in the venom in native crotoxin. The two crotapotins (Cdcolli III F3 and F4) differed in their ability to inhibit PLA2 activity, perhaps because of variations in their affinities for this enzyme. Cdcolli III F6 showed allosteric enzymatic behavior, with maximal activity at pH 8.3 and 36°C. Full PLA2 activity required the presence of a low Ca2+ concentration and was inhibited by Cu2+ and Zn2+ and by Cu2+ and Mg2+ in the presence and absence of Ca2+, respectively. These results indicate that crotoxin from C. d. collineatus venom is very similar enzymatically to crotoxin from C. d. terrificus. 21 3 131 136 Azevedo-Marques, M.M., Cupo, P., Coimbra, T.M., Hering, S.E., Rossi, M.A., Laure, C.J., (1982) Toxicon, 23, pp. 631-636 Beghini, D.G., Toyama, M.H., Hyslop, S., Sodek, L.C., Novello, J.C., Marangoni, S., (2000) J. Prot. Chem., 19, pp. 679-664 Bon, C., Changeux, J.P., Jeng, T.W., Fraenkel-Conrat, H., (1979) Eur. J. Biochem., 99, pp. 471-481 Bonfim, V.L., Toyama, M.H., Novello, J.C., Hyslop, S., Oliveira, C.R.B., Rodrigues-Simioni, L., Marangoni, S., (2001) J. Prot. Chem., 20, pp. 239-245 Breithaupt, H., (1976) Toxicon, 14, pp. 221-233 Cho, W., Kezdy, F.J., (1991) Methods Enzymol., 197, pp. 75-79 Cupo, P., Azevedo-Marques, M.M., Hering, S.E., (1988) Trans. R. Soc. Trop. Med. Hyg., 82, pp. 924-929 Faure, G., Bon, C., (1987) Toxicon, 25, pp. 229-234 Faure, G., Bon, C., (1988) Biochemistry, 27, pp. 730-738 Faure, G., Choumet, V., Bouchier, C., Camoin, L., Guillaume, J.L., Monegier, B., Vuilhorgne, M., Bon, C., (1994) Eur. J. Biochem., 223, pp. 161-164 Gutiérrez, J.M., Lomonte, B., (1995) Toxicon, 33, pp. 1405-1424 Habermann, E., Breithaupt, H., (1978) Toxicon, 16, pp. 19-30 Hendon, R.A., Fraenkel-Conrat, H., (1971) Proc. Natl. Acad. Sci. USA, 68, pp. 1560-1563 Holzer, M., Mackessy, S.P., (1996) Toxicon, 34, pp. 1149-1155 Landucci, E.C.T., Condino-Neto, A., Perez, A.C., Hyslop, S., Corrado, A.P., Novello, J.C., Marangoni, S., De Nucci, G., (1994) Toxicon, 32, pp. 217-226 Lennon, B.W., Kaiser, I., (1990) Comp. Biochem. Physiol. B., 97, pp. 695-699 Pieterson, W.A., Volwerck, J.J., De Haas, G.H., (1974) Biochemistry, 13, pp. 1439-1445 Rosenfeld, G., (1971) Venomous animals and their venoms, 2, pp. 345-362. , (Bücherl, W., and Buckley, E. E., eds), Academic Press, New York Rubsamen, K., Breithaupt, H., Habermann, E., (1971) Naunyn-Schmiedeberg's Arch. Pharmacol., 270, pp. 274-288 Schägger, H., Von Jagow, G., (1987) Anal. Biochem., 166, pp. 368-379 Toyama, M.H., Mancuso, L.C., Giglio, J.R., Novello, J.C., Oliveira, B., Marangoni, S., (1995) Biochem. Mol. Biol. Int., 37, pp. 1047-1055 Toyama, M.H., Carneiro, E.M., Marangoni, S., Barbosa, R.L., Corso, G., Boschero, A.C., (2000) Biochim. Biophys. Acta, 1474, pp. 56-60 Tu, A.T., Passey, R.B., Toom, P.M., (1970) Arch. Biochem. Biophys., 140, pp. 96-106