Artículos de revistas
In Vivo Degradation And Stimulating Effect Of Phaseolin On Nitrogen Secretion In Rats
Registro en:
Plant Foods For Human Nutrition. , v. 53, n. 3, p. 223 - 236, 1999.
9219668
10.1023/A:1008025922615
2-s2.0-0032847907
Autor
Santoro L.G.
Grant G.
Pusztai A.
Institución
Resumen
In short-term feeding experiments, about 78% of the phaseolin administered to rats was degraded regardless of the amounts of phaseolin intubated. In contrast, the total N found in the feces increased rapidly and exceeded the original administered amounts. The bulk of N output was not immunologically related to the glycoprotein. The effects of phaseolin on the stimulation of endogenous N secretion in the small intestine were confirmed from the results of acute experiments. Phaseolin fragments, derived from the breakdown of the native protein, when reapplied intragastrically to rats, were broken down further and to a similar extent as the original glycoprotein and were even more potent related to stimulation of N secretion. It is suggested that this secretagogue biological activity of phaseolin and not its resistance to gut proteolysis, is the main reason for the poor nutritional value of this glycoprotein. 53 3 223 236 Liener, I.E., Thompson, R.M., In vitro and in vivo studies on the digestibility of the major storage protein of navy bean (Phaseolus vulgaris) (1980) Qual Plant, Plant Foods Hum Nutr, 30, pp. 13-25 Deshpande, S.S., Nielsen, S.S., Nitrogenous constituents of selected grain legumes (1987) J Food Sci, 52, pp. 1321-1325 Santoro, L.G., Pusztai, A., Grant, G., Effects of short term feeding of rats with a highly purified phaseolin preparation (1997) Plant Foods Hum Nutr, 51, pp. 61-70 Palmer, R., McIntoch, A., Pusztai, A., The nutrition evaluation of kidney beans (Phaseolus vulgaris). The effect of nutritional value of seed germination and changes in trypsin inhibitor content (1973) J Sci Food Agric, 24, pp. 937-944 Puzstai, A., Duncan, I., Glycoprotein-I of Phaseolus vulgaris. Homogeneity and enzymatic properties (1971) Biochem Biophys Acta, 229, pp. 785-794 Pusztai, A., Stewart, J.C., Isolectins of Phaseolus vulgaris. Physicochemical studies (1978) Biochem Biophys Acta, 536, pp. 38-49 Harboe, N., Ingild, A., Quantitative immuno- electrophoresis (1973) Scand J Immunol, 1, pp. 161-164. , Axelsen NH, Kroll J, Week B (eds), Methods and applications Davidson, J., Mathieson, J., Boyne, A.W., The use of automation in determining nitrogen by the Kjeldahl method, with final calculations by computer (1970) Analyst, 95, pp. 181-193 Weeke, B., Rocket immunoelectrophoresis (1973) Scand J Immunol, 21, pp. 37-46. , Axelsen NH, Kroll J, Weeke B (eds), A Manual of Quantitative Immunoelectrophoresis. Methods and Applications Laemmli, U.K., Cleavage of structural proteins during assembly of the head of bacteriphage T4 (1970) Nature, 222, pp. 680-685 Santoro, L.G., (1988) Nutritional Utilization by Monogastric Animals of Glycoprotein-II (Phaseolin), the Major 7S Protein from Kidney Beans (Phaseolus Vulgaris). In Vivo and in Vitro Degradation of Glycoprotein-II by Rat Intestinal Proteases, , PhD Thesis. University of Aberdeen, Aberdeen, UK Santoro, L.G., Grant, G., Pusztai, A., Differences in the degradation in vivo and in vitro of phaseolin, the major storage protein of Phaseolus vulgaris seeds (1988) Biochem Soc Trans, 16, pp. 612-613 Vaintraub, I.A., Bassumer, R., Shutov, A.D., The action of trypsin and chymotrypsin on the reserve proteins of some leguminous seeds (1976) Nährung, 20, pp. 763-771 Bradbear, N., Boulter, D., The use of enzymic hydrolysis in vitro to study the digestibility of some Phaseolus seed proteins (1984) Qual Plant, Plant Foods Human Nutr, 34, pp. 3-13 Nielsen, S.S., Deshpande, S.S., Hermodson, M.A., Scott, M.P., Comparative digestibility of legume storage proteins (1988) J Agric Food Chem, 36, pp. 896-902 Chagas, E.P., (1993) Identificação e Caracterização Parcial de Albuminas e Globulinas de Três Cultivares Brasileiros de Phaseolus Vulgaris, , M.Sc. thesis. Universidade Estadual de Campinas, São Paulo, BR Oliveira, J.T.A., Pusztai, A., Grant, G., Changes in organs and tissues induced by feeding of purified Kidney bean (Phaseolus vulgaris) lectins (1988) Nutr Res, 8, pp. 943-947 Grant, G., Dorvard, P.M., Buchan, W., Armour, J.C., Pusztai, A., Consumption of diets containing raw soya beans (Glycine max), kidney beans (Phaseolus vulgaris), cowpeas (Vigna unguiculata) or lupin seeds (Lupinus angustufolius) by rats for up to 700 days: Effect on body composition and organ weights (1995) Br J Nutr, 73, pp. 17-29