Artículos de revistas
Molecular Characterization Of Sctfiiaγ, Encoding The Putative Tfiia Small Subunit From Sugarcane
Registro en:
Plant Cell Reports. , v. 29, n. 8, p. 857 - 864, 2010.
7217714
10.1007/s00299-010-0871-3
2-s2.0-77954535281
Autor
Gentile A.
da Cruz P.
Tavares R.G.
Krug-Baldacin M.G.
Menossi M.
Institución
Resumen
Transcription mediated by RNA polymerase II depends on a set of different transcription factors to form the pre-initiation complex. TFIIA is involved in the construction of this complex and increases the affinity of TBP for the DNA union region in vitro. In this study, we characterized the ScTFIIAγ gene, which encodes a homolog of the smaller subunit (γ) of transcription factor TFIIA in sugarcane. RNA blot analysis showed that ScTFIIAγ transcripts accumulate in all tissues evaluated, with higher levels in leaf roll and flowers. In situ hybridization showed that ScTFIIAγ was expressed in different cells of the reproductive meristem. In sugarcane plantlets, methyl jasmonate and absicic acid treatments as well as phosphate starvation had no influence on ScTFIIAγ transcript accumulation. The subcelullar localization assay demonstrates that ScTFIIAγ protein is directed to the cell nucleus. 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