Artículos de revistas
Protease Production During Growth And Autolysis Of Submerged Metarhizium Anisopliae Cultures
Registro en:
Revista De Microbiologia. , v. 30, n. 2, p. 107 - 113, 1999.
13714
2-s2.0-0344631735
Autor
Braga G.U.L.
Destefano; R.H.R.
Messias C.L.
Institución
Resumen
The growth and autolysis of two strains of the entomopathogenic deuteromycete fungus Metarhizium anisopliae var. anisopliae were evaluated in medium containing casein or glucose as carbon source. Parameters such as economic coefficient and degree of autolysis were determined for each strain. Protease production was determined throughout the growth and autolysis phases of the cultures on medium under conditions of protease induction (in the presence of casein as sole source of carbon and nitrogen). The fungus was shown to utilize casein as a carbon/energy source in a more efficient manner than glucose. The autolysis shown by the strains was intense under both types of growth conditions, reaching up to 62.7% of the dry mass produced and started soon after the depletion of the exogenous carbon source. The relationship between the proteolytic activities of the two strains evaluated varied significantly (a maximum of 19.78 on the 5th day and a minimum of 2.03 on the 16th day of growth) during the various growth and autolysis phases, clearly showing that the difference between the growth curves and the difference in the kinetics of enzyme production may decisively affect the process of strain selection for protease production. 30 2 107 113 Alfonso, C., Martinez, M.J., Reyes, F., Degradation of chitosan in the autolysis of mucorales (1991) Mycol. Res., 95, pp. 217-219 Braga, G.U.L., Messias, C.L., Vencovsky, R., Estimates of genetic parameters related to protease production by Metarhizium anisopliae (1994) J. Invertebr. Pathol., 64, pp. 6-12 Campbell, R.K., Perring, T.M., Barnes, G.L., Eikenbary, R.D., Gentry, C.R., Growth and sporulation of Beauveria bassiana and Metarhizium anisopliae on media containing various amino acids (1978) J. Invertebr. Pathol., 31, pp. 289-295 Goettel, M.S., St. Leger, R.J., Rizzo, N.W., Staples, R.C., Roberts, D.W., Ultrastructural localization of a cuticle-degrading protease produced by the entomopathogenic fungus Metarhizium anisopliae during penetration of host (Manduca sexta) cuticle (1989) J. Gen. Microbiol., 135, pp. 2233-2239 Kalisz, H.M., Moore, D., Wood, D.A., Protein utilization by basidiomycete fungi (1986) Trans. Br. Mycol. Soc., 86, pp. 519-525 Kucera, M., Proteases from the fungus Metarhizium anisopliae toxic for Galleria mellonella larvae (1980) J. Invertebr. Pathol., 35, pp. 304-310 Kucera, M., The production of toxic protease by the entomopathogenous fungus Metarhizium anisopliae in submerged culture (1981) J. Invertebr. Pathol., 38, pp. 33-38 Kucera, M., Partial purification and properties of Galleria mellonella larvae proteolytic inhibitors acting on Metarhizium anisopliae toxic protease (1984) J. Invertebr. Pathol., 43, pp. 190-196. , 1984 Lahoz, R., Ibeas, J.G., The autolysis of Aspergillus flavus in an alkaline medium (1968) J. Gen. Microbiol., 53, pp. 101-108 Lahoz, R., Miralles, M., Influence of the level of the carbon source on the autolysis of Aspergillus niger (1970) J. Gen. Microbiol., 60, pp. 271-276 Lahoz, R., Reyes, F., Martinez, M.J., Jimeno, L., Effect of the pH on the degree of autolysis of Aspergillus niger (1979) Can. J. Bot., 57, pp. 1901-1903 Nelson, N., A photometric adaptation of the Somogy method for the determination of glucose (1944) J. Biol. Chem., 153, pp. 375-380 Oliveira, N.T., Messias, C.L., Variability of strains of Metarhizium anisopliae Var. anisopliae (Metsch.) Sorokin in terms of protein content and spore germination (1996) Rev. Microbiol., 27, pp. 268-272 Pontecorvo, G., Roper, J.A., Hemmons, L.M., McDonald, K.F., Bufton, A.W.J., The genetics of Aspergillus nidulans (1953) Adv. Gen., 5, pp. 141-238 Reyes, F., Calatayud, J., Martinez, M.J., Chitinolytic activity in the autolysis of Aspergilus nidulans (1988) FEMS Microbiol. Lett., 49, pp. 239-243 Reyes, F., Lahoz, R., Variation in lysis of walls of Sclerotinia fructigena with age of culture (1977) J. Gen. Microbiol., 98, pp. 607-610 Reyes, F., Lahoz, R., Cornago, P., Autolysis of Neurospora crassa in different culture conditions and release of ß-N-Acetyl-Glucosaminidase and chitinase (1977) Trans. Br. Mycol. Soc., 68, pp. 357-361 Reyes, F., Lahoz, R., Moreno, A.V., Synthesis of 1,3-ß-glucanase and ß-N-acetylglucosaminidase during autolysis of Neurospora crassa (1981) J. Gen. Microbiol., 126, pp. 347-353 Rosato, Y.B., Messias, C.L., Azevedo, J.L., Production of extracellular enzymes by isolates of Metarhizium anisopliae (1981) J. Invertebr. Pathol., pp. 1-3 Santamaria, F., Reyes, F., Proteases produced during autolysis of filamentous fungi (1988) Trans. Br. Mycol. Soc., 91, pp. 217-220 Söderhäll, K., Unestam, T., Properties of extracellular enzymes from Amphanomyces astaci and their relevance in the penetration process of crayfish cuticle (1975) Physiol. Plant., 35, pp. 140-146 St. Leger, R.J., Charnley, A.K., Cooper, R.M., Characterization of cuticle-degrading proteases produced by the entomopathogen Metarhizium anisopliae (1987) Arch. Biochem. Biophys., 253, pp. 221-232 St. Leger, R.J., Cooper, R.M., Charnley, A.K., Production of cuticle-degrading enzymes by the entomopathogen Metarhizium anisopliae during infection of cuticles from Colliphora vomitoria and Manduca sexta (1987) J. Gen. Microbiol., 133, pp. 1371-1382