Artículos de revistas
Detection And Characterization Of Bacteriocin-producing Lactococcus Lactis Strains
Registro en:
Revista De Microbiologia. , v. 30, n. 2, p. 130 - 136, 1999.
13714
2-s2.0-0032720116
Autor
Moreno I.
Lerayer A.L.S.
De Freitas Leitao M.F.
Institución
Resumen
One hundred sixty seven strains of Lactococcus lactis were screened for bacteriocin production by well diffusion assay of GM17 agar. Fourteen (8.4%) produced antimicrobial activity other than organic acids, bacteriophages or hydrogen peroxide. The frequency of bacteriocin production ranged from 2% in L. lactis subsp. cremoris up to 12% in L. lactis subsp. lactis. Antimicrobial activities were not observed in any strain of L. lactis subsp. lactis var. diacetylactis. Among thirteen bacteriocin-producing strains and two nisin- producing strains (L. lactis subsp. lactis ATCC 11454 and L. lactis subsp. lactis CNRZ 150), eight (53%) were characterized as lactose-positive (Lac +) and proteinase-negative (Prt -). The bacteriocin-producing cultures were also characterized on the basis of plasmid content. All strains had 2 to 7 plasmids with molecular weights varying from 0.5 to 28.1 Mdal. 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