Artículos de revistas
Cephalosporin C Production And Genetic Improvement Of The Fungus Acremonium Chrysogenum Based On Morphological Mutant Isolation
Registro en:
Brazilian Journal Of Genetics. , v. 20, n. 2, p. 165 - 170, 1997.
1008455
2-s2.0-0030791918
Autor
Vialta A.
Catani C.F.
Junior R.B.
Azevedo J.L.
Institución
Resumen
C-10 strain of the fungus Acremonium chrysogenum produced about 1.5 g/l of cephalosporin C, a yield that was much lower than expected according to the literature. This discrepancy, confirmed by exhaustive tests, showed that C-10 strain lost its high cephalosporin C production ability, probably due to genetic events. Strain improvement, based on isolation of morphologically compact mutant colonies, confirmed that this is a useful procedure for isolation of strains with increased titer. About 2% of tested colonies produced significantly more cephalosporin C than the original strain. 20 2 165 170 Baldwin, J.E., Bird, J.W., Field, R.A., O'Calaghan, N., Schofield, C.J., Isolation and partial characterization of ACV synthetase from Cephalosporium ammonium and Streptomyces clavuligerus (1990) J. 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