Effect Of Fluoride-treated Enamel On Indirect Cytotoxicity Of A16% Carbamide Peroxide Bleaching Gel To Pulp Cells

dc.creatorSoares D.G.
dc.creatorRibeiro A.P.D.
dc.creatorLima A.F.
dc.creatorSacono N.T.
dc.creatorHebling J.
dc.creatorCosta C.A.S.
dc.date2013
dc.date2015-06-25T19:15:35Z
dc.date2015-11-26T15:13:10Z
dc.date2015-06-25T19:15:35Z
dc.date2015-11-26T15:13:10Z
dc.date.accessioned2018-03-28T22:23:17Z
dc.date.available2018-03-28T22:23:17Z
dc.identifier
dc.identifierBrazilian Dental Journal. , v. 24, n. 2, p. 121 - 127, 2013.
dc.identifier1036440
dc.identifier10.1590/0103-6440201302161
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84878463744&partnerID=40&md5=55224755e142d8568fefeeec8f2b2466
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/89280
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/89280
dc.identifier2-s2.0-84878463744
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1258572
dc.descriptionThe aim of this study was to evaluate the possibility of fluoride solutions applied to enamel to protect pulp cells against the trans-enamel and transdentinal cytotoxicity of a 16% carbamide peroxide (CP) bleaching gel. The CP gel was applied to enamel/ dentin discs adapted to artificial pulp chambers (8 h/day) during 1, 7 or 14 days, followed by fluoride (0.05% or 0.2%) application for 1 min. The extracts (culture medium in contact with dentin) were applied to MDPC-23 cells for 1 h, and cell metabolism (MTT assay), alkaline phosphatase (ALP) activity and cell membrane damage (flow cytometry) were analyzed. Knoop microhardness of enamel was also evaluated. Data were analyzed statistically by ANOVA and Kruskal-Wallis tests (a=0.05). For the MTT assay and ALP activity, significant reductions between the control and the bleached groups were observed (p<0.05). No statistically significant difference occurred among bleached groups (p>0.05), regardless of fluoride application or treatment days. Flow cytometry analysis demonstrated 30% of cell membrane damage in all bleached groups. After 14 days of treatment, the fluoride-treated enamel presented significantly higher microhardness values than the bleached-only group (p<0.05). It was concluded that, regardless of the increase in enamel hardness due to the application of fluoride solutions, the treated enamel surface did not prevent the toxic effects caused by the 16% CP gel to odontoblast-like cells.
dc.description24
dc.description2
dc.description121
dc.description127
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dc.languageen
dc.publisher
dc.relationBrazilian Dental Journal
dc.rightsaberto
dc.sourceScopus
dc.titleEffect Of Fluoride-treated Enamel On Indirect Cytotoxicity Of A16% Carbamide Peroxide Bleaching Gel To Pulp Cells
dc.typeArtículos de revistas


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