Artículos de revistas
Effect Of Nitrofen In The Final Stages Of Development Of The Diaphragm Muscle In Rats [efeito Do Nitrofen Na Fase Final Do Desenvolvimento Da Musculatura Do Diafragma Em Ratos]
Registro en:
Acta Cirurgica Brasileira. , v. 28, n. SUPPL.1, p. 13 - 18, 2013.
1028650
10.1590/S0102-86502013001300004
2-s2.0-84873665514
Autor
Lanhellas Goncalves F.L.
Oliveira F.S.
Schmidt A.F.
Pereira L.A.V.D.
Gallindo R.M.
Figueira R.L.
Sbragia L.
Institución
Resumen
PURPOSE: To evaluate the expression of myosin in muscle fibers of the diaphragm in experimental congenital diaphragmatic hernia (CDH). METHODS: Fetuses of pregnant rats were divided into four groups: External Control (EC), composed of non-manipulated rats; Nitrofen, composed of pregnant rats that received 100 mg of nitrofen (2,4-dichloro-4'nitrodiphenyl ether) diluted in olive oil on gestational day (GD) 9.5, whose fetuses developed CDH (N+) or not (N-), and Olive Oil Placebo (OO), composed of pregnant rats that received the oil on the same GD. The fetuses were collected on GD 18.5, 19.5, 20.5 and 21.5 (term = 22 days). We obtained body weight (BW) and photographed the diaphragm area (DA), hernia area (HA) and subsequent calculated the HA/DA ratio in N+ group. Samples of Diaphragm muscle were processed for histological staining with H/E and immunohistochemistry (IHQ) for myosin. RESULTS: The fetuses of N- and N+ groups had decreased BW and DA compared to EC and OO groups (p <0.001). HA was decreased on GD 18.5 compared to 21.5 (p <0.001) and the HA/DA ratio showed no difference. IHQ showed decreased expression of myosin in nitrofen groups. CONCLUSION: CDH induced by nitrofen model contributes to the understanding of muscularization in the formation of the diaphragm where the myosin expression is decreased. 28 SUPPL.1 13 18 Greer, J.J., Cote, D., Allan, D.W., Zhang, W., Babiuk, R.P., Ly, L., Lemke, R.P., Bagnall, K., Structure of the primordial diaphragm and defects associated with nitrofen-induced CDH (2000) J Appl Physiol, 89, pp. 2123-2129 Allan, D.W., Greer, J.J., Pathogenesis of nitrofen-induced congenital diaphragmatic hernia in fetal rats (1997) J Appl Physiol, 83, pp. 338-347 Stege, G., Fenton, A., Jaffray, B., Nihilism in the 1990s: The true mortality of congenital diafragmatic hernia (2003) Pediatrics, 112, pp. 532-535 Tannuri, U., Heart hypoplasia in an animal model of congenital diaphragmatic hernia (2001) Rev Hosp Clin Fac Med Sao Paulo, 56, pp. 173-178 Allan, D.W., Greer, J.J., Polysialylated NCAM expression during motor axon outgrowth and myogenesis in the fetal rat (1998) J Comp Neurol, 391, pp. 275-292 Brotto, M.A., Biesiadecki, B.J., Brotto, L.S., Nosek, T.M., Jin, J.P., Coupled expression of troponin T and troponin I isoforms in single skeletal muscle fibers correlates with contractility (2006) Am J Physiol Cell Physiol, 290, pp. C567-C576 Andersen, D.H., Incidence of congenital diaphragmatic in the young of rats bred on a diet deficient in vitamin A (1941) AM J Dis Child, 62, pp. 888-889 Schmidt, A.F., Gonçalves, F.L., Nassr, A.C., Pereira, L.A., Farmer, D., Sbragia, L., Antenatal steroid and tracheal occlusion restore vascular endothelial grouth factor receptors in congenital diaphragmatic hernia rat model (2010) Am J Obstet Gynecol, 203 (184), pp. e13-e20 Clugston, R.D., Zhang, W., Greer, J.J., Early development of the primordial mammalian diaphragm and cellular mechanisms of nitrofen-induced congenital diaphragmatic hernia (2010) Birth Defects Res a Clin Mol Teratol, 88, pp. 15-24 Dingemann, J., Doi, T., Ruttenstock, E., Puri, P., The role of primary myogenic regulatory factors in the developing diaphragmatic muscle in the nitrofen-induced diaphragmatic hernia (2011) Pediatr Surg Int, 27, pp. 579-582 Mayer, S., Metzger, R., Kluth, D., The embryology of the diaphragm (2011) Semin Pediatr Surg, 20, pp. 161-169 Tovar, J.A., Congenital diaphragmatic hernia (2012) Orphanet J Rare Dis, 7, p. 1 Babiuk, R.P., Greer, J.J., Diaphragm defects occur in a CDH hernia model independently of myogenesis and lung formation (2002) Am J Physiol Lung Cell Mol Physiol, 283, pp. 1310-1314 Gayan-Ramirez, G., Decramer, M., Clinical relevance of myosin isoforms in the diaphragm (2000) Rev Mal Respir, 17, pp. 574-584 Kelly, A.M., Rosser, B.W., Hoffman, R., Panettieri, R.A., Schiaffino, S., Rubinstein, N.A., Nemeth, P.M., Metabolic and contractile protein expression in developing rat diaphragm muscle (1991) J Neurosci, 11, pp. 1231-1242