Artículos de revistas
Production And Application Of Amylases Of Rhizopus Oryzae And Rhizopus Microsporus Var. Oligosporus From Industrial Waste In Acquisition Of Glucose
Registro en:
Chemical Papers. , v. 68, n. 4, p. 442 - 450, 2014.
3666352
10.2478/s11696-013-0466-x
2-s2.0-84891494494
Autor
De Freitas A.C.
Escaramboni B.
Carvalho A.F.A.
De Lima V.M.G.
De Oliva-Neto P.
Institución
Resumen
Amylases from Rhizopus oryzae and Rhizopus microsporus var. oligosporus were obtained using agro-industrial wastes as substrates in submerged batch cultures. The enzymatic complex was partially characterised for use in the production of glucose syrup. Type II wheat flour proved better than cassava bagasse as sole carbon source for amylase production. The optimum fermentation condition for both microorganisms was 96 hours at 30 C and the amylase thus produced was used for starch hydrolysis. The product of the enzymatic hydrolysis indicated that the enzyme obtained was glucoamylase, only glucose as final product was attained for both microorganisms. R. oligosporus was of greater interest than R. oryzae for amylase production, taking into account enzyme activity, cultivation time, thermal stability and pH range. Glucose syrup was produced using concentrated enzyme and 100 g L-1 starch in a 4 hours reaction at 50 C. The bioprocess studied can contribute to fungus glucoamylase production and application. © 2013 Institute of Chemistry, Slovak Academy of Sciences. 68 4 442 450 Anto, H., Trivedi, U., Patel, K., Alpha amylase production by Bacillus cereus MTCC 1305 using solid-state fermentation (2006) Food Technology and Biotechnology, 44 (2), pp. 241-245. , http://public.carnet.hr/ftbrfd/44-241.pdf Asgher, M., Asad, M.J., Rahman, S.U., Legge, R.L., A thermostable α-amylase from a moderately thermophilic Bacillus subtilis strain for starch processing (2007) Journal of Food Engineering, 79 (3), pp. 950-955. , DOI 10.1016/j.jfoodeng.2005.12.053, PII S0260877406002792 Belmessikh, A., Boukhalfa, H., Mechakra-Maza, A., Gheribi-Aoulmi, Z., Amrane, A., Statistical optimization of culture medium for neutral protease production by Aspergillus oryzae. Comparative study between solid and submerged fermentations on tomato pomace (2013) Journal of the Taiwan Institute of Chemical Engineers, 44, pp. 377-385. , 1:CAS:528:DC%2BC3sXhvFSlsb0%3D 10.1016/j.jtice.2012.12.011 Bradford, M.M., A rapid sensitive method for a quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Analytical Biochemistry, 72, pp. 248-254. , 1:CAS:528:DyaE28XksVehtrY%3D 10.1016/0003-2697(76)90527-3 Azevedo Carvalho, A.F., Zorzetto Goncalves, A., Da Silva, R., Gomes, E., A specific short dextrin-hydrolyzing extracellular glucosidase from the thermophilic fungus Thermoascus aurantiacus 179-5 (2006) Journal of Microbiology, 44 (3), pp. 276-283 Castro, A.M., Carvalho, D.F., Freire, D.M.G., Dos Reis Castilho, L., Economic analysis of the production of amylases and other hydrolases by Aspergillus awamori in solid-state fermentation of babassu cake (2010) Enzyme Research, 2010, p. 576872 De Souza, P.M., Magalhães, P.O., Application of microbial α-amylase in industry - A review (2010) Brazilian Journal of Microbiology, 41, pp. 850-861 Elisashvili, V., Kachlishvili, E., Penninckx, M., Effect of growth substrate, method of fermentation, and nitrogen source on lignocellulose-degrading enzymes production by white-rot basidiomycetes (2008) Journal of Industrial Microbiology & Biotechnology, 35, pp. 1531-1538. , 1:CAS:528:DC%2BD1cXht1eqtLvO 10.1007/s10295-008-0454-2 Ezeji, T.C., Bahl, H., Purification, characterization, and synergistic action of phytate-resistant α-amylase and α-glucosidase from Geobacillus thermodenitrificans HRO10 (2006) Journal of Biotechnology, 125 (1), pp. 27-38. , DOI 10.1016/j.jbiotec.2006.02.006, PII S0168165606001672 Franco, C.M.L., Daiuto, E.R., Demiate, I.M., Carvalho, L.J.C.B., Leonel, M., Cereda, M.P., Vilpoux, O.F., Sarmento, S.B.S., (2001) Propriedades Gerais Do Amido (Vol. 1, Pp. 141-185), , Fundação Cargill São Paulo, Brazil Gangadharan, D., Sivaramakrishnan, S., Amylolytic enzymes (2009) Biotechnology for Agro-industrial Residues Utilisation, pp. 359-369. , S N Nigan A. Pandey (eds) Springer Netherlands 10.1007/978-1-4020-9942-7- 19 Ghosh, B., Ray, R.R., Saccharification of raw native starches by extracellular isoamylase of Rhizopus oryzae (2010) Biotechnology, 9, pp. 224-228. , 1:CAS:528:DC%2BC3cXovVGjurk%3D 10.3923/biotech.2010.224.228 Ghosh, B., Ray, R., Current commercial perspective of Rhizopus oryzae: A review (2011) Journal of Applied Sciences, 14, pp. 2470-2486 Gomes, E., Guez, M.A.U., Martin, N., Da Silva, R., Enzimas termoestávies: Fontes, produção e aplicação industrial (2007) Química Nova, 30, pp. 136-145. , 1:CAS:528:DC%2BD2sXht1erurY%3D 10.1590/S0100-40422007000100025 Gupta, R., Gigras, P., Mohapatra, H., Goswami, V.K., Chauhan, B., Microbial α-amylases: A biotechnological perspective (2003) Process Biochemistry, 38 (11), pp. 1599-1616. , DOI 10.1016/S0032-9592(03)00053-0 Haq, I.-U., Ashraf, H., Iqbal, J., Qadeer, M.A., Production of alpha amylase by Bacillus licheniformis using an economical medium (2003) Bioresource Technology, 87 (1), pp. 57-61. , DOI 10.1016/S0960-8524(02)00198-0, PII S0960852402001980 Krishna, C., Solid-state fermentation systems - An overview (2005) Critical Reviews in Biotechnology, 25 (1-2), pp. 1-30. , DOI 10.1080/07388550590925383 Kumar, V., Sahai, V., Bisaria, V.S., Production of amylase and chlamydospores by Piriformospora indica, a root endophytic fungus (2012) Biocatalysis and Agricultural Biotechnology, 1, pp. 124-128. , 1:CAS:528:DC%2BC38Xmt12qsLk%3D 10.1016/j.bcab.2012.02.002 Liu, X.D., Xu, Y., A novel raw starch digesting α-amylase from a newly isolated Bacillus sp. YX-1: Purification and characterization (2008) Bioresource Technology, 99 (10), pp. 4315-4320. , DOI 10.1016/j.biortech.2007.08.040, PII S096085240700702X Michelin, M., Silva, T.M., Benassi, V.M., Peixoto-Nogueira, S.C., Moraes, L.A.B., Leão, J.M., Jorge, J.A., Polizeli, M.D.T.M., Purification and characterization of a thermostable α-amylase produced by the fungus Paecilomyces variotii (2010) Carbohydrate Research, 345, pp. 2348-2353. , 1:CAS:528:DC%2BC3cXhtlSmtL7O 10.1016/j.carres.2010.08.013 Mitidieri, S., Souza Martinelli, A.H., Schrank, A., Vainstein, M.H., Enzymatic detergent formulation containing amylase from Aspergillus niger. A comparative study with commercial detergent formulations (2006) Bioresource Technology, 97, pp. 1217-1224. , 1:CAS:528:DC%2BD28XjslCnsb4%3D 10.1016/j.biortech.2005.05.022 Mosier, N.S., Ladisch, M.R., (2009) Modern Biotechnology: Connecting Innovations in Microbiology and Biochemistry to Engineering Fundamentals, p. 433. , Wiley Hoboken, NJ, USA 10.1002/9780470473412 Nitayavardhana, S., Khanal, S.K., Biodiesel-derived crude glycerol bioconversion to animal feed: A sustainable option for a biodiesel refinery (2011) Bioresource Technology, 102, pp. 5808-5814. , 1:CAS:528:DC%2BC3MXlt1Cmt7s%3D 10.1016/j.biortech.2011.02.058 Norouzian, D., Akbarzadeh, A., Scharer, J.M., Moo Young, M., Fungal glucoamylases (2006) Biotechnology Advances, 24 (1), pp. 80-85. , DOI 10.1016/j.biotechadv.2005.06.003, PII S0734975005000765 Pandey, A., Nigam, P., Soccol, C.R., Soccol, V.T., Singh, D., Mohan, R., Advances in microbial amylases (2000) Biotechnology and Applied Biochemistry, 31 (2), pp. 135-152 Peixoto, S.C., Jorge, J.A., Terenzi, H.F., Polizeli, M.D.L.T.M., Rhizopus microsporus var rhizopodiformis: A thermotolerant fungus with potential for production of thermostable amylases (2003) International Microbiology, 6 (4), pp. 269-273. , DOI 10.1007/s10123-003-0140-1 Rani, R., Ghosh, S., Production of phytase under solid-state fermentation using Rhizopus oryzae: Novel strain improvement approach and studies on purification and characterization (2011) Bioresource Technology, 102, pp. 10641-10649. , 1:CAS:528:DC%2BC3MXhtlKrt7bP 10.1016/j.biortech.2011.08.075 Sarrouh, B., Santos, T.M., Miyoshi, A., Dias, R., Azevedo, V., Up-to-date insight on industrial enzymes applications and global market (2012) Journal of Bioprocessing & Biotechniques, 4, p. 002 Sharma, A., Satyanarayana, T., Microbial acid-stable α-amylases: Characteristics, genetic engineering and applications (2013) Process Biochemistry, 48, pp. 201-211. , 1:CAS:528:DC%2BC3sXht1Omtrg%3D 10.1016/j.procbio.2012.12.018 Sivaramakrishnan, S., Gangadharan, D., Nampoothiri, K.M., Soccol, C.R., Pandey, A., α-Amylases from microbial sources - An overview on recent developments (2006) Food Technology and Biotechnology, 44 (2), pp. 173-184. , http://public.carnet.hr/ftbrfd/44-173.pdf Soccol, C.R., Iloki, I., Marin, B., Raimbault, M., Comparative production of alpha-amylase, glucoamylase and protein enrichment of raw and cooked cassava by Rhizopus strains in submerged and solid state fermentations (1994) Journal of Food Science and Technology, 31, pp. 320-323. , 1:CAS:528:DyaK2MXhvVGisLs%3D Soccol, C.R., Vandenberghe, L.P.S., Overview of applied solid-state fermentation in Brazil (2003) Biochemical Engineering Journal, 13 (2-3), pp. 205-218. , DOI 10.1016/S1369-703X(02)00133-X, PII S1369703X0200133X Soccol, C.R., Rojan, P.J., Patel, A.K., Woiciechowski, A.L., Vandenberghe, L.P.S., Pandey, A., Glucoamylase (2006) Enzyme Technology, pp. 221-230. , A. Pandey C. Webb C. R Soccol C. Larroche (eds) Asiatec Publishers New Delhi, India Soni, S.K., Kaur, A., Gupta, J.K., A solid state fermentation based bacterial α-amylase and fungal glucoamylase system and its suitability for the hydrolysis of wheat starch (2003) Process Biochemistry, 39 (2), pp. 185-192. , DOI 10.1016/S0032-9592(03)00058-X Surmely, R., Alvarez, H., Cereda, M.P., Vilpoux, O.F., Hidrólise do amido (2003) Tecnologia, Usos e Potencialidades de Tuberosas Amiláceas Latino Americanas, 3, pp. 376-449 Van Der Maarel, M.J.E.C., Van Der Veen, B., Uitdehaag, J.C.M., Leemhuis, H., Dijkhuizen, L., Properties and applications of starch-converting enzymes of the α-amylase family (2002) Journal of Biotechnology, 94 (2), pp. 137-155. , DOI 10.1016/S0168-1656(01)00407-2, PII S0168165601004072 Van Leeuwen, J., Rasmussen, M.L., Sankaran, S., Koza, C.R., Erickson, D.T., Mitra, D., Jin, B., Fungal treatment of crop processing wastewaters with value-added coproducts (2012) Sustainable Bioenergy and Bioproducts, Green Energy and Technology, pp. 13-44. , K. Gopalakrishnan J. van Leeuwen R. C Brown (eds) Springer London, UK 10.1007/978-1-4471-2324-8-2