Artículos de revistas
Angiogenic Effects Of Cryosurgery With Liquid Nitrogen On The Normal Skin Of Rats, Through Morphometric Study
Registro en:
Anais Brasileiros De Dermatologia. Sociedade Brasileira De Dermatologia, v. 89, n. 3, p. 410 - 413, 2014.
3650596
10.1590/abd1806-4841.20142249
2-s2.0-84902580562
Autor
Pimentel C.B.
de Moraes A.M.
Cintra M.L.
Institución
Resumen
BACKGROUND: Cryosurgery is an efficient therapeutic technique used to treat benign and malignant cutaneous diseases. The primary active mechanism of cryosurgery is related to vascular effects on treated tissue. After a cryosurgical procedure, exuberant granulation tissue is formed at the injection site, probably as a result of angiogenic stimulation of the cryogen and inflammatory response, particularly in endothelial cells. OBJECTIVE: To evaluate the angiogenic effects of freezing, as part of the phenomenon of healing rat skin subjected to previous injury. METHODS: Two incisions were made in each of the twenty rats, which were divided randomly into two groups of ten. After 3 days, cryosurgery with liquid nitrogen was performed in one of incisions. The rats' samples were then collected, cut and stained to conduct histopathological examination, to assess the local angiogenesis in differing moments and situations. RESULTS: It was possible to demonstrate that cryosurgery, in spite of promoting cell death and accentuated local inflammation soon after its application, induces quicker cell proliferation in the affected tissue and maintenance of this rate in a second phase, than in tissue healing without this procedure. CONCLUSIONS: These findings, together with the knowledge that there is a direct relationship between mononuclear cells and neovascularization (the development of a rich system of new vessels in injury caused by cold), suggest that cryosurgery possesses angiogenic stimulus, even though complete healing takes longer to occur. The significance level for statistical tests was 5% (p<0,05). © 2014 by Anais Brasileiros de Dermatologia. 89 3 410 413 Lizhi, N., Kecheng, X., Feng, M., Cryosurgery for lung cancer (2012) J. Thorac. Dis, 4 (4), pp. 408-416 Lucas, R., (2001) Avaliacao E Comparacao De Diferentes Tecnicas De Criocirurgia Com Nitrogenio Liquido Em Caes Da Raca Beagle, Pela Tomografia Computadorizada, Histologia E Imunohistoquimica [tese], , Sao Paulo (SP): Universidade de Sao Paulo Poziomczyk, C.S., Koche, B., Dornelles Mde, A., Dornelles, S.I., Pain evaluation in the cryosurgery of actinic keratoses (2011) An Bras Dermatol, 86, pp. 645-650 Gage, A.A., History of cryosurgery (1998) Semin Surg Oncol, 14, pp. 99-109 Gage, A.A., Baust, J., Mechanisms of tissue injury in cryosurgery (1998) Cryobiology, 37, pp. 171-186 Yamaguchi, Y., Yoshikawa, K., Cutaneous Wound healing: An update (2001) J Dermatol, 28, pp. 521-534 Belman, S., Adams-Ray, J., Vascular reactions after experimental cold injury (1956) Angiology, 7, pp. 339-367 Carmeliet, P., Angiogenesis in health and disease (2003) Nat Med, 9, pp. 653-660 Calvin, M., Cutaneous wound repair (1998) Wounds, 10, pp. 12-32 Fox, S.B., Harris, A.L., Histological quantitation of tumor angiogenesis (2004) APMIS, 112, pp. 413-430 Fina, L., Molgaard, H.V., Robertson, D., Bradley, N.J., Monaghan, P., Delia, D., Expression of the CD34 gene in vascular endothelial cells (1990) Blood, 75, pp. 2417-2426