Artículos de revistas
Comparison Of The Solution Of Histidine-tryptophan-alfacetoglutarate With Histidine-tryptophan-glutamate As Cardioplegic Agents In Isolated Rat Hearts: An Immunohistochemical Study [comparação Da Solução De Histidina-triptofano-alfacetoglutarato Com Histidina-triptofano-glutamato Como Agentes Cardioplégicos Em Corações Isolados De Ratos: Estudo Imuno-histoquímico]
Registro en:
Brazilian Journal Of Cardiovascular Surgery. Sociedade Brasileira De Cirurgia Cardiovascular, v. 29, n. 1, p. 83 - 88, 2014.
1027638
10.5935/1678-9741.20140015
2-s2.0-84901936176
Autor
de Oliveira M.A.B.
Ferreira L.C.
de Campos Zuccari D.A.P.
Brandi A.C.
dos Santos C.A.
Botelho P.H.H.
Petrucci O.
Braile D.M.
Institución
Resumen
Introduction: Cardiac arrest during heart surgery is a common procedure and allows the surgeon to perform surgical procedures in an environment free of blood and movement. Using a model of isolated rat heart, the authors compare a new cardioplegic solution containing histidine-tryptophan-glutamate (group 2) with the histidine-tryptophan-alphacetoglutarate (group 1) routinely used by some cardiac surgeons. Objective: To assess caspase, IL-8 and KI-67 in isolated rat hearts using immunohistochemistry. Methods: 20 Wistar male rats were anesthetized and heparinized. The chest was opened, cardioctomy was performed and 40 ml/kg of the appropriate cardioplegic solution was infused. The hearts were kept for 2 hours at 4°C in the same solution, and thereafter, placed in the Langendorff apparatus for 30 minutes with Ringer-Locke solution. Immunohistochemistry analysis of caspase, IL-8, and KI-67 were performed. 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