Artículos de revistas
State Of The Art Two-dimensional Liquid Chromatography: Fundamental Concepts, Instrumentation, And Applications [o Estado Da Arte Da Cromatografia Líquida Bidimensional: Conceitos Fundamentais, Instrumentac¸ão E Aplicac¸ões]
Registro en:
Quimica Nova. Sociedade Brasileira De Quimica, v. 37, n. 10, p. 1680 - 1691, 2014.
1004042
10.5935/0100-4042.20140261
2-s2.0-84916234741
Autor
Mogollon N.G.S.
De Lima P.F.
Gama M.R.
Furlan M.F.
Braga S.C.G.N.
Prata P.S.
Jardim I.C.S.F.
Augusto F.
Institución
Resumen
The constant evolution of science and the growing demand for new technologies have led to new techniques in instrumentation that can improve detection, separation, resolution, and peak capacity. Comprehensive two-dimensional liquid chromatography (LCxLC) is presented as a powerful tool in complex sample analyses. During an analysis, a sample is subjected to two independent separation mechanisms that are combined, resulting in increased resolving power. For appropriate application of LCxLC, understanding the influence of parameters that require optimization is necessary. The main purpose of optimization is to predict the combination of stationary phases, separation conditions, and instrumental requirements to obtain the best separation performance. This review discusses theoretical, intrumental, and chemometric aspects of LCxLC and focuses on its applications in foods. 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