Artículos de revistas
Hypertrophic Adenoid Is A Major Infection Site Of Human Bocavirus 1
Registro en:
Journal Of Clinical Microbiology. American Society For Microbiology, v. 52, n. 8, p. 3030 - 3037, 2014.
951137
10.1128/JCM.00870-14
2-s2.0-84905234043
Autor
Proenca-Modena J.L.
Paula F.E.
Buzatto G.P.
Carenzi L.R.
Saturno T.H.
Prates M.C.
Silva M.L.
Delcaro L.S.
Valera F.C.P.
Tamashiro E.
Anselmo-Lima W.T.
Arruda E.
Institución
Resumen
Human bocavirus 1 (HBoV1) is associated with respiratory infections worldwide, mainly in children. Similar to other parvoviruses, it is believed that HBoV1 can persist for long periods of time in humans, probably through maintaining concatemers of the virus single-stranded DNA genome in the nuclei of infected cells. Recently, HBoV-1 was detected in high rates in adenoid and palatine tonsils samples from patients with chronic adenotonsillar diseases, but nothing is known about the virus replication levels in those tissues. A 3-year prospective hospital-based study was conducted to detect and quantify HBoV1 DNA and mRNAs in samples of the adenoids (AD), palatine tonsils (PT), nasopharyngeal secretions (NPS), and peripheral blood (PB) from patients undergoing tonsillectomy for tonsillar hypertrophy or recurrent tonsillitis. HBoV1 was detected in 25.3% of the AD samples, while the rates of detection in the PT, NPS, and PB samples were 7.2%, 10.5%, and 1.7%, respectively. The viral loads were higher in AD samples, and 27.3% of the patients with HBoV had mRNA detectable in this tissue. High viral loads and detectable mRNA in the AD were associated with HBoV1 detection in the other sample sites. The adenoids are an important site of HBoV1 replication and persistence in children with tonsillar hypertrophy. The adenoids contain high HBoV1 loads and are frequently positive for HBoV mRNA, and this is associated with the detection of HBoV1 in secretions. Copyright © 2014, American Society for Microbiology. All Rights Reserved. 52 8 3030 3037 Allander, T., Tammi, M.T., Eriksson, M., Bjerkner, A., Tiveljung-Lindell, A., Andersson, B., Cloning of a human parvovirus by molecular screening of respiratory tract samples (2005) Proceedings of the National Academy of Sciences of the United States of America, 102 (36), pp. 12891-12896. , DOI 10.1073/pnas.0504666102 Schildgen, O., Müller, A., Allander, T., Mackay, I.M., Völz, S., Kupfer, B., Simon, A., Human bocavirus: Passenger or pathogen in acute respiratory tract infections? (2008) Clin. Microbiol. Rev., 21, pp. 291-304. , http://dx.doi.org/10.1128/CMR.00030-07, table of contents Arthur, J.L., Higgins, G.D., Davidson, G.P., Givney, R.C., Ratcliff, R.M., A novel bocavirus associated with acute gastroenteritis in Australian children (2009) PLoS Pathog., 5, pp. e1000391. , http://dx.doi.org/10.1371/journal.ppat.1000391 Kapoor, A., Simmonds, P., Slikas, E., Li, L., Bodhidatta, L., Sethabutr, O., Triki, H., Delwart, E., Human bocaviruses are highly diverse, dispersed, recombination prone, and prevalent in enteric infections (2010) J. Infect. Dis., 201, pp. 1633-1643. , http://dx.doi.org/10.1086/652416 Kapoor, A., Slikas, E., Simmonds, P., Chieochansin, T., Naeem, A., Shaukat, S., Alam, M.M., Delwart, E., A newly identified bocavirus species in human stool (2009) J. Infect. Dis., 199, pp. 196-200. , http://dx.doi.org/10.1086/595831 Proença-Modena, J.L., Gagliardi, T.B., Da Paula, F.E., Iwamoto, M.A., Criado, M.F., Camara, A.A., Acrani, G.O., Arruda, E., Detection of human bocavirus mRNA in respiratory secretions correlates with high viral load and concurrent diarrhea (2011) PLoS One, 6, pp. e21083. , http://dx.doi.org/10.1371/journal.pone.0021083 Proenca-Modena, J.L., Martinez, M., Amarilla, A.A., Espínola, E.E., Galeano, M.E., Fariña, N., Russomando, G., Arruda, E., Viral load of human bocavirus-1 in stools from children with viral diarrhoea in Paraguay (2013) Epidemiol. Infect., 141, pp. 2576-2580. , http://dx.doi.org/10.1017/S095026881300023X Allander, T., Jartti, T., Gupta, S., Niesters, H.G.M., Lehtinen, P., Osterback, R., Vuorinen, T., Ruuskanen, O., Human bocavirus and acute wheezing in children (2007) Clinical Infectious Diseases, 44 (7), pp. 904-910. , DOI 10.1086/512196 Christensen, A., Døllner, H., Skake, L.H., Krokstad, S., Moe, N., Nordbø, S.A., Detection of spliced mRNA from human bocavirus 1 in clinical samples from children with respiratory tract infections (2013) Emerg. Infect. Dis., 19, pp. 574-580. , http://dx.doi.org/10.3201/eid1904.121775 Christensen, A., Nordbø, S.A., Krokstad, S., Rognlien, A.G., Døllner, H., Human bocavirus in children: Mono-detection, high viral load and viraemia are associated with respiratory tract infection (2010) J. Clin. Virol., 49, pp. 158-162. , http://dx.doi.org/10.1016/j.jcv.2010.07.016 Nascimento-Carvalho, C.M., Cardoso, M.R., Meriluoto, M., Kemppainen, K., Kantola, K., Ruuskanen, O., Hedman, K., Söderlund-Venermo, M., Human bocavirus infection diagnosed serologically among children admitted to hospital with community-acquired pneumonia in a tropical region (2012) J. Med. Virol., 84, pp. 253-258. , http://dx.doi.org/10.1002/jmv.22268 Deng, X., Yan, Z., Luo, Y., Xu, J., Cheng, F., Li, Y., Engelhardt, F., Qiu, J., In vitro modeling of human bocavirus 1 infection of polarized primary human airway epithelia (2013) J. Virol., 87, pp. 4097-4102. , http://dx.doi.org/10.1128/JVI.03132-12 Lüsebrink, J., Schildgen, V., Tillmann, R.L., Wittleben, F., Böhmer, A., Müller, A., Schildgen, O., Detection of head-to-tail DNA sequences of human bocavirus in clinical samples (2011) PLoS One, 6, pp. e19457. , http://dx.doi.org/10.1371/journal.pone.0019457 Kapoor, A., Hornig, M., Asokan, A., Williams, B., Henriquez, J.A., Lipkin, W.I., Bocavirus episome in infected human tissue contains non-identical termini (2011) PLoS One, 6, pp. e21362. , http://dx.doi.org/10.1371/journal.pone.0021362 Zhao, H., Zhao, L., Sun, Y., Qian, Y., Liu, L., Jia, L., Zhang, Y., Dong, H., Detection of a bocavirus circular genome in fecal specimens from children with acute diarrhea in Beijing, China (2012) PLoS One, 7, pp. e48980. , http://dx.doi.org/10.1371/journal.pone.0048980 Windisch, W., Schildgen, V., Malecki, M., Lenz, J., Brockmann, M., Karagiannidis, C., Schildgen, O., Detection of HBoV DNA in idiopathic lung fibrosis, Cologne, Germany (2013) J. Clin. Virol., 58, pp. 325-327. , http://dx.doi.org/10.1016/j.jcv.2013.05.020 Nave, H., Gebert, A., Pabst, R., Morphology and immunology of the human palatine tonsil (2001) Anatomy and Embryology, 204 (5), pp. 367-373. , DOI 10.1007/s004290100210 Garnett, C.T., Erdman, D., Xu, W., Gooding, L.R., Prevalence and quantitation of species C adenovirus DNA in human mucosal lymphocytes (2002) Journal of Virology, 76 (21), pp. 10608-10616. , DOI 10.1128/JVI.76.21.10608-10616.2002 Garnett, C.T., Talekar, G., Mahr, J.A., Huang, W., Zhang, Y., Ornelles, D.A., Gooding, L.R., Latent species C adenoviruses in human tonsil tissues (2009) J. Virol., 83, pp. 2417-2428. , http://dx.doi.org/10.1128/JVI.02392-08 Hislop, A.D., Kuo, M., Drake-Lee, A.B., Akbar, A.N., Bergler, W., Hammerschmitt, N., Khan, N., Rickinson, A.B., Tonsillar homing of Epstein-Barr virus-specific CD8+ T cells and the virus-host balance (2005) Journal of Clinical Investigation, 115 (9), pp. 2546-2555. , DOI 10.1172/JCI24810 Lu, X., Gooding, L.R., Erdman, D.D., Human bocavirus in tonsillar lymphocytes (2008) Emerg. Infect. Dis., 14, pp. 1332-1334. , http://dx.doi.org/10.3201/eid1408.080300 Proenca-Modena, J.L., Valera, F.C.P., Jacob, M.G., Buzatto, G.P., Saturno, T.H., Lopes, L., Souza, J.M., Anselmo-Lima, W.T., High rates of detection of respiratory viruses in tonsillar tissues from children with chronic adenotonsillar disease (2012) PLoS One, 7, pp. e42136. , http://dx.doi.org/10.1371/journal.pone.0042136 Paradise, J.L., Bluestone, C.D., Bachman, R.Z., Efficacy of tonsillectomy for recurrent throat infection in severely affected children. Results of parallel randomized and nonrandomized clinical trials (1984) New England Journal of Medicine, 310 (11), pp. 674-683 Neske, F., Blessing, K., Tollmann, F., Schubert, J., Rethwilm, A., Kreth, H.W., Weissbrich, B., Real-time PCR for diagnosis of human bocavirus infections and phylogenetic analysis (2007) Journal of Clinical Microbiology, 45 (7), pp. 2116-2122. , DOI 10.1128/JCM.00027-07 Huang, Q., Deng, X., Yan, Z., Cheng, F., Luo, Y., Shen, W., Lei-Butters, D.C., Qiu, J., Establishment of a reverse genetics system for studying human bocavirus in human airway epithelia (2012) PLoS Pathog., 8, pp. e1002899. , http://dx.doi.org/10.1371/journal.ppat.1002899 Deng, X., Li, Y., Qiu, J., Human bocavirus 1 infects commercially available primary human airway epithelium cultures productively (2014) J. Virol. Methods, 195, pp. 112-119. , http://dx.doi.org/10.1016/j.jviromet.2013.10.012 Martin, E.T., Fairchok, M.P., Kuypers, J., Magaret, A., Zerr, D.M., Wald, A., Englund, J.A., Frequent and prolonged shedding of bocavirus in young children attending daycare (2010) J. Infect. Dis., 201, pp. 1625-1632. , http://dx.doi.org/10.1086/652405 Schildgen, V., Malecki, M., Tillmann, R.L., Brockmann, M., Schildgen, O., The human bocavirus is associated with some lung and colorectal cancers and persists in solid tumors (2013) PLoS One, 8, pp. e68020. , http://dx.doi.org/10.1371/journal.pone.0068020 Monroe, K.M., Yang, Z., Johnson, J.R., Geng, X., Doitsh, G., Krogan, N.J., Greene, W.C., IFI16 DNA sensor is required for death of lymphoid CD4 T cells abortively infected with HIV (2014) Science, 343, pp. 428-432. , http://dx.doi.org/10.1126/science.1243640 Orzalli, M.H., Conwell, S.E., Berrios, C., DeCaprio, J.A., Knipe, D.M., Nuclear interferon-inducible protein 16 promotes silencing of herpesviral and transfected DNA (2013) Proc. Natl. Acad. Sci. U. S. A., 110, pp. E4492-E4501. , http://dx.doi.org/10.1073/pnas.1316194110 Szalmás, A., Papp, Z., Csomor, P., Kónya, J., Sziklai, I., Szekanecz, Z., Karosi, T., Microbiological profile of adenoid hypertrophy correlates to clinical diagnosis in children (2013) Biomed. Res. Int., 2013, p. 629607. , http://dx.doi.org/10.1155/2013/629607