Actas de congresos
The Interactions Between Non-thermal Atmospheric Pressure Plasma And Ex-vivo Dermal Fibroblasts
Registro en:
Procedia Engineering. , v. 59, n. , p. 92 - 100, 2013.
18777058
10.1016/j.proeng.2013.05.098
2-s2.0-84891681002
Autor
Lopes B.B.
De Paula Leite Kraft M.B.
Rehder J.
Batista F.R.X.
Puzzi M.B.
Institución
Resumen
The cold atmospheric plasma (CAPs) is the fourth state of matter, which means that it is an ionized gas, obtained at room temperature and atmospheric pressure when energy is supplied to a gas. Its composition is complex, involving chemical (gas's molecules, free radicals, ions and electrons) and physical (Ultra-violet radiation and electric field) components. It has been successfully used in the industry for the processing of materials and the recent discovery of its efficacy in sterilization of microorganisms has trigged a large quantity of research in the biomedical field. The expectations of good that the CAPs may bring to medical treatments are high and therefore this study analyzes the effects caused by cold atmospheric pressure plasma in ex-vivo dermal fibroblasts. For that, fibroblast cell culture was obtained from ex-vivo human skin and treated with different conditions of CAPs, such as treatment type (direct or indirect). This work aimed to determine if ex-vivo human fibroblasts remain viable after these treatments. For that, we counted the number of cells right after the experiments and compared it with the control, which did not receive any kind of plasma. For further results we also counted the number of viable cells 3 and 5 days after the application. The fibroblasts were treated with 85W plasma, from 30 seconds up to 60 seconds. Our findings show that CAPs obtained from Argon gas can be applied to in-house human fibroblasts culture directly and indirectly without altering the cells' viability. It does not cause apoptosis in all of the cells treated nor does it exacerbate its proliferation. Further studies need to be done in order to analyze if it alters the cells' functionality. © 2013 The Authors. 59
92 100 European Community on Computational Methods,in Applied Sciences (ECCOMAS) Bian, X., Chen, Q., Zhang, Y., Sang, L., Tang, W., Deposition of nano-diamond-like carbon films by an atmospheric pressure plasma gun and diagnostic by optical emission spectrum on the process (2008) Surface & Coatings Technology, 202 (2008), pp. 5383-5385 Subasri, R., Jyothirmayi, A., Reddy, D.S., Effect of plasma surface treatment and heat treatment ambience on mechanical and corrosion protection properties of hybrid sol-gel coatings on aluminum (2010) Surface & Coatings Technology, 205 (2010), pp. 806-813 Laroussi, M., Sterilization of contaminated matter with an atmospheric pressure plasma (1996) IEEE Trans. Plasma Sci., 24, pp. 1188-1191 Crookes, W., On radiant matter spectroscopy: A new method of spectrum analysis (1883) Proc Roy Soc, 35, pp. 262-271. , 1883 Chen, F.F., Industrial applications of low-temperature plasma physics (1995) Physical Plasmas, 2 (6) Weller, R., Nitric oxide - A newly discovered chemical transmitter in human skin (1997) Br. J. Dermatol., 137, pp. 665-672 Heinlin, J., Isbary, G., Stolz, W., Morfill, G., Landthaler, M., Shimizu, T., Seffes, B., Karrer, S., Plasma applications in medicine with a special focus on dermatology (2011) J Eur Acad Dermatol Venereol., (25), pp. 1-11. , 2011 Stoffels, E., Sakiyama, Y., Graves, D., Cold atmospheric plasma: Charged species and their interactions with cells and tissues (2008) IEEE Trans. Plasma Sci., 36 (4), pp. 1441-1457 Heinlin, J., Isbary, G., Stolz, W., Morfill, G., Landthaler, M., Shimizu, T., Seffes, B., Karrer, S., Plasma medicine: Possible applications in dermatology (2010) Journal of the German Society of Dermatology, 12. , 2010 (band 8) Ehlbeck, J., Schnabel, U., Polak, M., Winter, J., Von Woedtke, T.H., Brandenburg, R., Von Dem Hagen, T., Weltmann, K.D., Low temperature atmospheric pressure plasma sources for microbial decontamination (2011) J. Phys. D: Appl. Phys., 44 (2011), p. 013002. , (18pp) Bogle, M., Arndt, K., Dover, J., Evaluation of plasma skin regeneration technology in low-energy full-facial rejuvenation (2007) Arch. Dermatol., 143 (2), pp. 168-174. , 2007 Fridman, G., Shereshevsky, A., Peddinghaus, M., Gutsol, A., Vasilets, V., Brooks, A., Balasubramanian, M., Fridman, A., Bio-medical applications of non-thermal atmospheric pressure plasma (2006) 37th AIAA Plasmadynamics and Lasers Conference, , 2006 San Francisco, California Stoffels, E., Sladek, R., Kieft, I., Gas plasma effets on living cells (2004) Physica Scripta, 107, pp. 79-84 Fridman, G., Friedman, G., Gutsol, A., Shekhter, A.B., Vasilets, V.N., Fridman, A., Applied plasma medicine (2008) Plasma Process and Polymers, 5, pp. 503-533. , 2008 Moisan, M., Barbeau, J., Moreau, S., Pelletier, J., Tabrizian, M., Yahia, L.H., Low-temperature sterilization using gas plasmas: A review of the experiments and an analysis of the inactivation mechanisms (2001) International Journal of Pharmacy, 226, pp. 1-21 Reuter, S., Niemi, K., Gathen, V., Döbele, H., Generation of atomic oxygen in the effluent of an atmospheric pressure plasma jet (2009) Plasma Sources Sciences. Technologies, 18, pp. 1-9 Reuter, S., Tresp, H., Wende, K., Hammer, M., Winter, J., Masur, K., Schimidt-Blecker, A., Weltmann, K., From RONS to ROS: Tailoring plasma jet treatment of skin cells (2012) IEEE Transactions on Plasma Science, 40 (11), pp. 2986-2994 Pipa, A., Reuter, S., Foest, R., Weltmann, K., Controlling the NO production of an atmospheric pressure plasma jet Journal of Physics D: Applied Physics, 45, pp. 1-7 Rehder, J., Souto, L.R., Issa, C.M.B.M., Puzzi, M.B., Model of human epidermis reconstructed in vitro with keratinocytes and melanocytes on dead de-epidermized human dermis (2004) Sao Paulo Med J, 122, pp. 22-25. , (2004) Fridman, G., Peddinghaus, M., Ayan, H., Fridman, A., Balasubramanian, M., Gutsol, A., Brooks, A., Friedman, G., Blood coagulation and living tissue sterilization by floating-electrode dielectric barrier discharge in air (2006) Plasma Chemistry: Plasma Process, 26 (4), pp. 425-442 Stoffels, E., Kieft, I., Sladek, R., Van Den Bedem, L., Van Der Laan, E., Steinbuch, M., Plasma needle for in vivo medical treatment: Recent developments and perspectives (2006) Plasma Sources Sciences and Technologies, 15 (4), pp. 169-180 Stolz, W., Low-temperature argon plasma for the sterilization of chronic wounds: From bench to bedside (2007) 1st International Conference in Plasma Medicine, pp. 15-18 Fridman, G., Brooks, G., Balasubramanian, A., Fridman, A., Gutsol, A., Vasilets, V., Ayan, H., Friedman, G., Comparison of direct and indirect effects of non-thermal atmospheric-pressure plasma on bacteria (2007) Plasma Proces and Polymers, 4 (4), pp. 370-375 Fridman, G., Shereshevsky, A., Jost, M., Brooks, A., Fridman, A., Gutsol, A., Vasilets, V., Friedman, G., Floating electrode dielectric barrier discharge plasma in air promoting apoptotic behavior in melanoma skin cancer cell lines (2007) Plasma Chemistry and Plasma Proces., 27 (2), pp. 163-176 Kieft, I., Kurdi, M., Stoffels, E., Reattachment and apoptosis after plasma-needle treatment of cultured cells (2006) IEEE Transactions. on Plasma Science, 34 (4), pp. 1331-1336 Stoffels, E., Roks, A., Deelman, E., Delayed effects of cold plasma treatment on vascular cells (2008) Plasma Process And. Polymers, 5 (6), pp. 599-605 Liebmann, J., Scherer, J., Bibinov, N., Rajasekaram, P., Kovacs, R., Gesche, R., Awakowicz, P., Kolb-Bachofen, V., Biological effects of nitric oxide generated by an atmospheric pressure gas-plasma on human skin cells (2011) Nitric Oxide, 24 (1), pp. 8-16. , 2011. Jan1 Tan, F., O'Neill, F., Naciri, M., Dowling, D., Al-Rubeai, M., Cellular and transcriptomic analys of human mesenchymal stem cell response to plasma-activated hydroxyapatite coating (2012) Acta Biomater, , (2012) doi: 10.1016/j.actbio.2011.12.014 Stoffels, E., Sakiyama, Y., Graves, D.B., Cold atmospheric plasma: Charged sprecies and their interactions with cells and tissues (2008) IEEE Transactions on Plasma Science, 36 (4). , August 2008 Tümmel, S., Mertens, N., Wang, J., Viöl, W., Low temperature plasma treatment of living human cells (2007) Plasma Process. Polym., 4, pp. S465-469. , 2007 Haertel, B., Wende, K., Von Woedtke, T., Weltmann, K.D., Lindequist, U., Non-thermal atmospheric pressure plasma can influence cell adhesion molecules on HaCaT-keratinocytes (2010) Experimental Dermatology, 20, pp. 278-296. , John Wiley & Sons A/S Yan, X., Zou, F., Zhao, S., Lu, Z., He, G., Xiong, Z., Xiong, Q., Yang, G., On the mechanism of plasma inducing cell apoptosis (2010) IEEE Transactions on Plasma Science, 38 (9). , September 2010 Hoentsch, M., Von Woedtke, T., Weltmann, K.D., Nebe, J.B., Time-dependent effects of low-temperature atmospheric pressure pargon plasma on epithelial cell attachment, viability and tight junction formation in vitro (2012) J. Phys. D: Appl. Phys., 45 (2012), p. 025206. , (9pp) Chapman, S., Liu, X., Meyers, C., Schlegel, R., McBride, A., Human keratinocytes are efficiently immortalized by a rho kinase inhibitor (2010) The Journal of Clinical Investigation, 120 (7), pp. 2619-2626