Actas de congresos
Il-12 Enhances Proliferation Of Peripheral Blood Mononuclear Cells From Chagas' Disease Patients To Trypanosoma Cruzi Antigen
Registro en:
Immunology Letters. , v. 57, n. 01/03/15, p. 39 - 45, 1997.
1652478
10.1016/S0165-2478(97)00079-5
2-s2.0-0030793046
Autor
De Barros-Mazon S.
Guariento M.E.
De Almeida Abrahamsohn I.A.
Institución
Resumen
Chagas' disease is caused by infection with Trypanasoma cruzi. Patients in the chronic phase of infection were grouped as belonging to the asymptomatic (or indeterminate), cardiac and cardiac plus digestive forms. Previous studies have described abnormal immune responsiveness by peripheral blood mononuclear cells (PBMC) from chronic chagasic patients. We report significant parasite antigen (T-Ag)-stimulated PBMC proliferative responses to be present in all three groups of patients. Treatment of T-Ag-stimulated cultures with rIL-12 significantly amplifies proliferative responses in all patients' groups, with similar rates of increment. IL-12 enhances T-Ag- specific lymphoproliferation without increasing proliferation of unstimulated PBMC from normal individuals or from patients. Comparatively, treatment with rIL-2 enhances both T-Ag-specific and unstimulated proliferation by PBMC from patients and normals. Thus, IL-12 acts on pre-activated ceils while IL-2 also stimulates resting cells. No synergism was obtained by the combined use of IL-12 and IL-2. Therefore IL-12 can act as a more selective amplifier of T. cruzi reactive cells than IL-2. IL-12, by enhancing parasite-antigen specific immunity, could be of potential therapeutic use to control reactivated T. cruzi infections concomitant to AIDS or other situations of immunosuppression. 57 01/03/15 39 45 Teixeira, A.R.L., Teixeira, G., Macedo, V., Prata, A., (1978) J. Clin. Invest., 62, pp. 1132-1141 Voltarelli, J.C., Donadi, E.A., Falcão, R.P., (1987) Trans. R. Soc. Trop. Med. Hyg., 81, pp. 169-170 Montufar, O.M.B., Musatti, C.C., Mendes, E., Mendes, N.F., (1977) J. Clin. Microbiol., 5, pp. 401-404 De Titto, E.H., Segura, E.L., Braun, M., (1983) Immunol. Lett., 6, pp. 161-167 Mosca, W., Plaja, J., Hubsch, R., Cedillos, R., (1985) J. Clin. Microbiol., 22, pp. 438-441 Morato, M.J.F., Brener, Z., Cançado, J.R., Nunes, R.M.B., Chiari, E., Gazzinelli, G., (1986) Am. J. Trop. Med. Hyg., 35, pp. 505-511 Cetron, M.S., Basilio, F.P., Moraes, A.P., Sousa, A.Q., Paes, J.N., Kahn, S.J., Wener, M.H., Van Voorhis, W.C., (1993) Am. J. Trop. Med. Hyg., 49, pp. 370-382 Colley, D.G., Howard, J.M., (1991) Jpn. J. Trop. Med. Hyg., 19, pp. 175-190 Stracieri, A.B.P.L., Voltarelli, J.C., (1995) Parasite Immunol., 17, pp. 381-383 Brodskyn, C.I., Barral, A., Bulhões, M.A., Souto, T., Machado, W.C., Barral-Netto, M., (1996) Clin. Exp. Immunol., 105, pp. 450-455 Dutra, W.O., Martins-Filho, O.A., Cançado, J.R., Pinto-Dias, J.C., Brener, Z., Freeman, G.L., Colley, D.G., Parra, J.C., (1994) Int. Immunol., 6, pp. 499-506 Dutra, W.O., Martins-Filho, O.A., Cançado, J.R., Pinto-Dias, J.C., Brener, Z., Gazzinelli, G., Carvalho, J.F., Colley, D.G., (1996) Scand. J. Immunol., 43, pp. 88-93 Dutra, W.O., Gollob, K.J., Pinto-Dias, J.C., Gazzinelli, G., Correa-Oliveira, R., Coffman, R.L., Carvalho-Parra, J.F., (1997) Scand. J. Immunol., 45, pp. 74-80 Sartori, A.M., Lopes, M.H., Caramelli, B., Duarte, M.I.K., Pinto, P.L., Amato-Neto, V., Shikanai-Yasuda, M.A., (1995) Clin. Infect. Dis., 21, pp. 1297-1299 Rocha, A., De-Meneses, A.C., Da-Silva, A.M., Ferreira, M.S., Nishioka, S.A., Burgarelli, M.K., Almeida, E., Lopes, E.R., (1994) Am. J. Trop. Med. Hyg., 50, pp. 261-268 Biron, C.A., Gazzinelli, R.T., (1995) Curr. Opin. Immunol., 7, pp. 485-496 Hall, S.S., (1995) Science, 268, pp. 1432-1434 Abrahamsohn, I.A., Coffman, R.L., (1996) Exp. Parasitol., 84, pp. 231-244 Aliberti, J.C.S., Cardoso, M.A.G., Martins, G.A., Gazzinelli, R.T., Vieira, L.Q., Suva, J.S., (1996) Infection and Immunity, 64, pp. 1961-1967 Barbosa-De-Oliveira, L.C., Curotto-De-Lafaille, M.A., Lima, G.M.C., Abrahamsohn, I.A., (1996) Cell. Immunol., 170, pp. 41-53 Stern, A.S., Podlaski, F.J., Mulmes, J.D., Pan, Y.-C.E., Quinn, P.M., Wolitzky, A.G., Familletti, P.C., Gately, M.K., (1990) Proc. Natl. Acad. Sci. USA, 87, pp. 6808-6812 Abrahamsohn, I.A., Coffman, R.L., (1995) J. Immunol., 155, pp. 3955-3963 Gately, M.K., Desai, B.B., Wolitzky, A.G., Quinn, P.M., Dwyer, C.M., Podlaski, F.J., Familletti, P.C., Stern, A.S., (1991) J. Immunol., 147, pp. 874-882 Perussia, B., Chan, S., D'Andrea, A., Tsuji, K., Santoli, D., Pospisil, M., Young, D., Trinchieri, G., (1992) J. Immunol., 149, pp. 3495-3502 Caligiuri, M.A., Zmuidzinas, A., Manley, T.J., Levine, H., Smith, K.A., Ritz, J., (1990) J. Exp. Med., 171, pp. 1509-1524 Gusmão, R.D., Rassi, A., Rezende, I.M., Neva, F.A., (1984) Am. J. Trop. Med. Hyg., 33, pp. 827-834 Trinchieri, G., (1995) Annu. Rev. Immunol., 13, pp. 251-276 Wu, C.Y., Demeure, C., Kiniwa, M., Gately, M., Delespesse, G., (1993) J. Immunol., 151, pp. 1938-1949 Meyaard, L., Hovenkamp, E., Otto, S.A., Miedema, F., (1996) J. Immunol., 156, pp. 2776-2782 Jeannin, P., Delneste, Y., Seveso, M., Life, P., Bonnefoy, J.-Y., (1996) J. Immunol., 156, pp. 3159-3165