Artículos de revistas
Neutrophils Of Rheumatoid Arthritis Patients On Anti-tnf-α Therapy And In Disease Remission Present Reduced Adhesive Functions In Association With Decreased Circulating Neutrophil-attractant Chemokine Levels
Registro en:
Scandinavian Journal Of Immunology. , v. 73, n. 4, p. 309 - 318, 2011.
3009475
10.1111/j.1365-3083.2011.02503.x
2-s2.0-79952061870
Autor
Dominical V.M.
Bertolo M.B.
Almeida C.B.
Garrido V.T.
Miguel L.I.
Costa F.F.
Conran N.
Institución
Resumen
Neutrophils participate in the initiation and progression of rheumatoid arthritis (RA) although the exact mechanisms responsible for neutrophil accumulation in rheumatoid joints are not understood. This study compared the adhesive and chemotactic functions of neutrophils from RA patients in activity (DAS28>3.2) and not in activity (DAS28<2.6) and observed the effects of different treatment approaches on these functions. Neutrophils were isolated from healthy controls (CON), and patients with active or inactive RA in use of therapy not specific for RA (NSAIDs), in use of DMARDs and in use of anti-TNF-α therapy. Adhesive and chemotactic properties were evaluated using in vitro assays; adhesion molecule expression was assessed by flow cytometry and real-time PCR and circulating chemokines were determined by ELISA. No significant alterations in the adhesive and chemotactic properties of neutrophils from active RA were observed when compared to CON neutrophils, independently of treatment regimen. In contrast, neutrophils from RA patients in disease remission presented reduced adhesive properties and a lower spontaneous chemotactic capacity, in association with decreased adhesion molecule expression, although profiles of alterations differed for those patients on DMARDs and those on anti-TNF-α therapy. Circulating levels of the major neutrophilic chemokines, IL-8 and epithelial neutrophil activating peptide-78, were also significantly decreased in those patients demonstrating a clinical response. Remission of RA appears to be associated with ameliorations in aspects important for neutrophil adhesion and chemotaxis; whether these alterations contribute to decrease neutrophil migration to the synovial fluid, with consequent improvements in the clinical manifestations of RA, remains to be determined. © 2011 The Authors. Scandinavian Journal of Immunology © 2011 Blackwell Publishing Ltd. 73 4 309 318 Gaffo, A., Saag, K.G., Curtis, J.R., Treatment of rheumatoid arthritis (2006) Am J Health Syst Pharm, 63, pp. 2451-65 Stanczyk, J., Kowalski, M.L., Grzegorczyk, J., RANTES and chemotactic activity in synovial fluids from patients with rheumatoid arthritis and osteoarthritis (2005) Mediators Inflamm, 2005, pp. 343-8 Wright, H.L., Moots, R.J., Bucknall, R.C., Edwards, S.W., Neutrophil function in inflammation and inflammatory diseases (2010) Rheumatology (Oxford), 49, pp. 1618-31 Cascao, R., Rosario, H.S., Souto-Carneiro, M.M., Fonseca, J.E., Neutrophils in rheumatoid arthritis: more than simple final effectors (2010) Autoimmun Rev, 9, pp. 531-5 Cross, A., Bakstad, D., Allen, J.C., Neutrophil gene expression in rheumatoid arthritis (2005) Pathophysiology, 12, pp. 191-202 Tarrant, T.K., Patel, D.D., Chemokines and leukocyte trafficking in rheumatoid arthritis (2006) Pathophysiology, 13, pp. 1-14 Grespan, R., Fukada, S.Y., Lemos, H.P., CXCR2-specific chemokines mediate leukotriene B4-dependent recruitment of neutrophils to inflamed joints in mice with antigen-induced arthritis (2008) Arthritis Rheum, 58, pp. 2030-40 Lemos, H.P., Grespan, R., Vieira, S.M., Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production (2009) Proc Natl Acad Sci USA, 106, pp. 5954-9 Eyles, J.L., Hickey, M.J., Norman, M.U., A key role for G-CSF-induced neutrophil production and trafficking during inflammatory arthritis (2008) Blood, 112, pp. 5193-201 Cross, A., Bucknall, R.C., Cassatella, M.A., Edwards, S.W., Moots, R.J., Synovial fluid neutrophils transcribe and express class II major histocompatibility complex molecules in rheumatoid arthritis (2003) Arthritis Rheum, 48, pp. 2796-806 Cassatella, M.A., The production of cytokines by polymorphonuclear neutrophils (1995) Immunol Today, 16, pp. 21-6 Burgos, R.A., Hidalgo, M.A., Figueroa, C.D., Conejeros, I., Hancke, J.L., New potential targets to modulate neutrophil function in inflammation (2009) Mini Rev Med Chem, 9, pp. 153-68 Mpofu, S., Fatima, F., Moots, R.J., Anti-TNF-alpha therapies: they are all the same (aren't they?) (2005) Rheumatology, 44, pp. 271-3 Capsoni, F., Sarzi-Puttini, P., Atzeni, F., Effect of adalimumab on neutrophil function in patients with rheumatoid arthritis (2005) Arthritis Res Ther, 7, pp. R250-5 Arnett, F.C., Edworthy, S.M., Bloch, D.A., The American Rheumatism Association 1987 revised criteria for the classification of rheumatoid arthritis (1988) Arthritis Rheum, 31, pp. 315-24 English, D., Andersen BR Single-step separation of red blood cells. Granulocytes and mononuclear leukocytes on discontinuous density gradients of Ficoll-Hypaque (1974) J Immunol Methods, 5, pp. 249-52 Assis, A., Conran, N., Canalli, A.A., Effect of cytokines and chemokines on sickle neutrophil adhesion to fibronectin (2005) Acta Haematol, 113, pp. 130-6 Bradley, P.P., Priebat, D.A., Christensen, R.D., Rothstein, G., Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker (1982) J Invest Dermatol, 78, pp. 206-9 Gambero, S., Canalli, A.A., Traina, F., Therapy with hydroxyurea is associated with reduced adhesion molecule gene and protein expression in sickle red cells with a concomitant reduction in adhesive properties (2007) Eur J Haematol, 78, pp. 144-51 Vandesompele, J., De Preter, K., Pattyn, F., Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes (2002) Genome Biol, 3, pp. 341-3411 Ley, K., Laudanna, C., Cybulsky, M.I., Nourshargh, S., Getting to the site of inflammation: the leukocyte adhesion cascade updated (2007) Nat Rev Immunol, 7, pp. 678-89 Howe, G.B., Fordham, J.N., Brown, K.A., Currey, H.L., Polymorphonuclear cell function in rheumatoid arthritis and in Felty's syndrome (1981) Ann Rheum Dis, 40, pp. 370-5 Sheehan, N.J., Brown, K.A., Perry, J.D., Adherence of rheumatoid polymorphonuclear cells (PMNs) to cultured endothelial cell monolayers (1987) Ann Rheum Dis, 46, pp. 93-7 Hashimoto, H., Yamamura, M., Nishiya, K., Ota, Z., Impaired interleukin-8-dependent chemotaxis by synovial fluid polymorphonuclear leukocytes in rheumatoid arthritis (1994) Acta Med Okayama, 48, pp. 181-7 Vollmer, K.L., Alberts, J.S., Carper, H.T., Mandell, G.L., Tumor necrosis factor-alpha decreases neutrophil chemotaxis to N-formyl-1-methionyl-1-leucyl-1-phenylalanine: analysis of single cell movement (1992) J Leukoc Biol, 52, pp. 630-6 Verri Jr, W.A., Souto, F.O., Vieira, S.M., IL-33 induces neutrophil migration in rheumatoid arthritis and is a target of anti-TNF therapy (2010) Ann Rheum Dis, 69, pp. 1697-703 Bond, A., Hay, F.C., L-selectin expression on the surface of peripheral blood leucocytes from rheumatoid arthritis patients is linked to disease activity (1997) Scand J Immunol, 46, pp. 312-6 Smalley, D.M., Ley, K., L-selectin: mechanisms and physiological significance of ectodomain cleavage (2005) J Cell Mol Med, 9, pp. 255-66 Lee, D., Caputo, K.E., Hammer, D.A., King, M.R., Adhesive dynamics simulations of the mechanical shedding of L-selectin from the neutrophil surface (2009) J Theor Biol, 260, pp. 27-30 Eniola-Adefeso, O., Huang, R.B., Smith, C.W., Kinetics of LFA-1 mediated adhesion of human neutrophils to ICAM-1--role of E-selectin signaling post-activation (2009) Ann Biomed Eng, 37, pp. 737-48 Hogg, N., Leitinger, B., Shape and shift changes related to the function of leukocyte integrins LFA-1 and Mac-1 (2001) J Leukoc Biol, 69, pp. 893-8 Klimiuk, P.A., Sierakowski, S., Domyslawska, I., Chwiecko, J., Regulation of serum chemokines following infliximab therapy in patients with rheumatoid arthritis (2006) Clin Exp Rheumatol, 24, pp. 529-33 Lun, S.W., Wong, C.K., Tam, L.S., Li, E.K., Lam, C.W., Decreased ex vivo production of TNF-alpha and IL-8 by peripheral blood cells of patients with rheumatoid arthritis after infliximab therapy (2007) Int Immunopharmacol, 7, pp. 1668-77 Walz, A., Schmutz, P., Mueller, C., Schnyder-Candrian, S., Regulation and function of the CXC chemokine ENA-78 in monocytes and its role in disease (1997) J Leukoc Biol, 62, pp. 604-11 Koch, A.E., Kunkel, S.L., Harlow, L.A., Epithelial neutrophil activating peptide-78: a novel chemotactic cytokine for neutrophils in arthritis (1994) J Clin Invest, 94, pp. 1012-8 Wessels, J.A., Huizinga, T.W., Guchelaar, H.J., Recent insights in the pharmacological actions of methotrexate in the treatment of rheumatoid arthritis (2008) Rheumatology, 47, pp. 249-55 Taylor, P.C., Pharmacology of TNF blockade in rheumatoid arthritis and other chronic inflammatory diseases (2010) Curr Opin Pharmacol, 10, pp. 308-15