Artículos de revistas
Analysis Of Virulence Genes In Escherichia Coli Isolated From Grated Cheese [pesquisa De Genes De Virulência Em Escherichia Coli Isolada De Queijo Ralado]
Registro en:
Ciencia E Tecnologia De Alimentos. , v. 31, n. 1, p. 106 - 108, 2011.
1012061
10.1590/S0101-20612011000100014
2-s2.0-79957466436
Autor
Martins R.P.
Nakazato L.
Dutra V.
Leite D.S.
Institución
Resumen
This research aimed to verify the presence of virulence genes in strains of Escherichia coli isolated from grated cheese sold in farmers' markets of Cuiabá-MT, Brazil. Forty samples of this food were submitted for microbiological analysis and 22 (55%) tested positive for E. coli. Next, 64 strains of E. coli were isolated from the positive samples and screened by the polymerase chain reaction (PCR) for the presence of the genes encoding the following virulence factors: stx1 and stx2 (verotoxin types 1 and 2), eae (intimin), lt1 (heat-labile toxin type 1), st1 (heat-stable toxin type 1), cnf1 and cnf2 (cytotoxic necrozing factor types 1 and 2), and cdtB (cytolethal distending toxin). All the isolates were negative for the genes stx1, stx2, eae, lt1, st1, cnf1, and cdtB, and five strains (7.81%) were positive for cnf2. A low prevalence of E. coli positive for virulence factors associated with the pathogenesis of diarrhoea was observed in this study. However, the presence of CNF-2 producing strains and the possibility of occurrence and scattering of other virulence factors that were not surveyed in the work indicate the risk related to the consumption of grated cheese from farmers' markets. 31 1 106 108 Beutin, L., Identification of human-pathogenic strains of shiga toxin-producing Escherichia coli from food by a combination of serotyping and molecular typing of shiga toxin genes (2007) Applied and Environmental Microbiology, 73 (15), pp. 4769-4775 Blanco, M., Distribution and characterization of faecal necrotoxigenic Escherichia coli CNF1+ and CNF2+ isolated from healthy cows and calves (1998) Veterinary Microbiology, 59, pp. 183-192 Brant, L.M.F., Avaliação da qualidade microbiológica do queijo-de-minas artesanal do Serro-MG (2007) Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 59 (6), pp. 1570-1574 Regulamento Técnico para Fixação de Identidade e Qualidade de Queijo Ralado (1997) Diário Oficial [da] República Federativa do Brasil, p. 1968. , BRASIL. Portaria n. 357, de 04 de Setembro de 1997, Brasília, DF, 08 set, Seção 1 Clark, C.G., PCR for detection of cdt-III and the relative frequencies of cytolethal distending toxin variant-producing Escherichia coli isolates from humans and cattle (2002) Journal of Clinical Microbiology, 40 (7), pp. 2671-2674 Cobbold, R., Desmarchelier, P., Characterisation and clonal relationships of Shiga-toxigenic Escherichia coli (STEC) isolated from Australian dairy cattle (2001) Veterinary Microbiology, 79, pp. 323-335 Conedera, G., Verocytotoxin-producing Escherichia coli O157 in minced beef and dairy products in Italy (2004) International Journal of Food Microbiology, 96, pp. 67-73 Gonzalez, A.G.M., Enteropathogenicity markers in Escherichia coli strains isolated from soft white cheese and poultry in Rio de Janeiro, Brazil (2000) Food Microbiology, 17, pp. 321-328 Holt, J.G., Krieg, N.R., (1984) Bergey's manual of systematic bacteriology, , Baltimore: Williams & Wilkins Le Magrex-Debar, E., Evaluation of biohazards in dehydrated biofilms on foodstuff packaging (2000) International Journal of Food Microbiology, 55, pp. 239-243 Loguercio, A.P., Aleixo, J.A.G., Microbiologia de queijo tipo minas frescal produzido artesanalmente (2001) Ciência rural, 31 (6), pp. 1063-1067 Paneto, Occurrence of toxigenic Escherichia coli in raw milk cheese in Brazil (2007) Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 59 (2), pp. 508-512 Pimentel, E.F., Avaliação da rotulagem e da qualidade físicoquímica e microbiológica de queijo ralado (2002) Ciência e Tecnologia de Alimentos, 22 (3), pp. 289-294 Quinto, E.J., Cepeda, A., Incidence of toxigenic Escherichia coli in soft cheese made with raw of pasteurized milk (1997) Letters in Applied Microbiology, 24, pp. 291-295 Ribeiro, M.G., Fator necrosante citotóxico em Escherichia coli isolada de mastite clínica bovina (2002) Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 54 (6), pp. 648-650 Salvadori, M.R., Virulence factors of Escherichia coli isolated from calves with diarrhoea in Brazil (2003) Brazilian Journal of Microbiology, 34, pp. 230-235 Silva, A.S., Leite, D.S., Investigation of putative CDT gene in Escherichia coli isolates from pigs with diarrhea (2002) Veterinary Microbiology, 89, pp. 195-199 Toledo, M.R.F., Fontes, C.F., Trabulsi, L.R., EPM-Modificação do meio Rugai e Araújo para realização simultânea dos testes de produção de gás a partir da glicose, H2S, urease e triptofano desaminase (1982) Revista de Microbiologia, 13, pp. 309-315. , São Paulo Toledo, M.R.F., Fontes, C.F., Trabulsi, L.R., Um meio para a realização dos testes de motilidade, indol e lisina descarboxilase (1982) Revista de Microbiologia, 13, pp. 230-235 Zaffari, C.B., Mello, J.F., Costa, M., Qualidade bacteriológica de queijos artesanais comercializados em estradas do litoral norte do Rio Grande do Sul, Brasil (2007) Ciência Rural, 37 (3), pp. 862-867