Artículos de revistas
Effects Of The Topical Application Of Hydroalcoholic Leaf Extract Of Oncidium Flexuosum Sims. (orchidaceae) And Microcurrent On The Healing Of Wounds Surgically Induced In Wistar Rats
Registro en:
Evidence-based Complementary And Alternative Medicine. , v. 2011, n. , p. - , 2011.
1741427X
10.1155/2011/950347
2-s2.0-80052692602
Autor
De Gaspi F.O.D.G.
Foglio M.A.
De Carvalho J.E.
Santos G.M.T.
Testa M.
Passarini Jr. J.R.
De Moraes C.P.
Esquisatto M.A.M.
Mendonca J.S.
Mendonca F.A.S.
Institución
Resumen
This study evaluated the wound healing activity of hydroalcoholic leaf extract of Oncidium flexuosum Sims. (Orchidaceae), an important native plant of Brazil, combined or not with microcurrent stimulation. Wistar rats were randomly divided into four groups of nine animals: control (C), topical application of the extract (OF), treated with a microcurrent (10 μA/2min) (MC), and topical application of the extract plus microcurrent (OF + MC). Tissue samples were obtained 2, 6, and 10 days after injury and submitted to structural and morphometric analysis. The simultaneous application of OF + MC was found to be highly effective in terms of the parameters analyzed (P<.05), with positive effects on the area of newly formed tissue, number of fibroblasts, number of newly formed blood vessels, and epithelial thickness. Morphometric data confirmed the structural findings. The O. flexuosum leaf extract contains active compounds that speed the healing process, especially when applied simultaneously with microcurrent stimulation. Copyright © 2011 Fernanda Oliveira de G. de Gaspi et al. 2011
Kumar, B., Vijayakumar, M., Govindarajan, R., Pushpangadan, P., Ethnopharmacological approaches to wound healing-Exploring medicinal plants of India (2007) Journal of Ethnopharmacology, 114 (2), pp. 103-113. , DOI 10.1016/j.jep.2007.08.010, PII S0378874107004126 Rao, S.G., Udupa, A.L., Udupa, S.L., Calendula and hypericum: Two homeopathic drugs promoting wound healing in rats (1991) Fitoterapia, 62 (6), pp. 508-510 Schempp, C.M., Winghofer, B., Ludtke, R., Simon-Haarhaus, B., Schopf, E., Simon, J.C., Topical application of St John's wort (Hypericum perforatum L.) and of its metabolite hyperforin inhibits the allostimulatory capacity of epidermal cells (2000) British Journal of Dermatology, 142 (5), pp. 979-984. , DOI 10.1046/j.1365-2133.2000.03482.x Martins, P.S., Alves, A.L.G., Hussni, C.A., Sequeira, J.L., Nicoletti, J.L.M., Thomassian, A., Comparao entre fitoterpicos de uso tpico na cicatrizao de pele em eqinos (2003) Archives of Veterinary Science, 8 (2), pp. 1-7 Joly, A.B., (1993) BotnicaIntroduo Taxonomia Vegetal, , 11th Companhia Editora Nacional Raven, P.H., Everest, R.F., Eichhorn, S.E., (2001) Biologia Vegetal, , 6th Guanabara Koogan Pabst, G.J., Estudos no gnero Oncidium Sw. (Orchidaceae)-IV (1972) Bradea, 1 (15), pp. 137-143 Garay, L.A., Stacy, J.E., Synopsis of the genus Oncidium (1974) Bradea, 1 (40), pp. 393-427 Dressler, R.L., (1990) The Orchids: Natural History and Classification, , Harvard University Press Arditti, J., (1992) Fundamentals of Orchid Biology, , New York, NY, USA John Wiley Kong, J.M., Goh, N.K., Chia, L.S., Chia, T.F., Recent advances in traditional plant drugs and orchids (2003) Acta Pharmacologica Sinica, 24 (1), pp. 7-21 (1975) Herbal Pharmacology in the People's Republic of China, , Washington, DC, USA National Academy of Sciences Ward, H., (1936) Herbal Manual: The Medicinal, Toilet, Culinary and Other Uses of 130 of the Most Commonly Used Herbs, , London, UK LN Fowler Co Cheng, N., Van Hoof, H., Bockx, E., The effects of electric currents on ATP generation, protein synthesis, and membrane transport in rat skin (1982) Clinical Orthopaedics and Related Research, 171, pp. 264-272 Houghton, P.E., Kincaid, C.B., Lovell, M., Campbell, K.E., Keast, D.H., Woodbury, M.G., Harris, K.A., Effect of electrical stimulation on chronic leg ulcer size and appearance (2003) Physical Therapy, 83 (1), pp. 17-28 Kloth, L.C., Feedar, J.A., Acceleration of wound healing with high voltage, monophasic, pulsed current (1988) Physical Therapy, 68 (4), pp. 503-508 Kloth, L.C., Electrical stimulation for wound healing: A review of evidence from in vitro studies, animal experiments, and clinical trials (2005) International Journal of Lower Extremity Wounds, 4 (1), pp. 23-44. , DOI 10.1177/1534734605275733 Bayat, M., Asgari-Moghadam, Z., Maroufi, M., Rezaie, F.-S., Bayat, M., Rakhshan, M., Experimental wound healing using microamperage electrical stimulation in rabbits (2006) Journal of Rehabilitation Research and Development, 43 (2), pp. 219-226. , http://www.vard.org/jour/06/43/2/pdf/bayat.pdf, DOI 10.1682/JRRD.2005.05.0089 Santos, V.N.S., Ferreira, L.M., Horibe, E.K., Duarte, I.S., Electric microcurrent in the restoration of the skin undergone a trichloroacetic acid peeling in rats (2004) Acta Cirurgica Brasileira, 19 (5), pp. 466-470 Valle, K.K.R., Reis, L.L., Bouvent, J.J., Shida, C.S., Efeito da aplicao de microcorrente elétrica na restaurao de pele de ratos exposta ao de radicais livres Proceedings of the 21th Congresso Brasileiro de Engenharia Biomédica 2008, pp. 247-249 Harborne, J.B., (1984) Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis, , 2nd Chapman and Hall Marini-Bettlo, G.B., Nicoletti, M., Patamia, M., Plant screening by chemical and chromatographic procedures under field conditions (1981) Journal of Chromatography, 218, pp. 113-217 Matos, F.J.A., (1997) Introduo Fitoqumica Experimental, , Fortaleza, Brazil Edies UFC International Guiding Principles for Biomedical Research Involving Animals, , CIOMS, 1985 (1996) Guide for the Care and Use of Laboratory Animals, , NIH Mustoe, T.A., Pierce, G.F., Thomason, A., Accelerated healing of incisional wounds in rats induced by transforming growth factor-β (1987) Science, 237 (4820), pp. 1333-1336 Mendona, F.A.S., Passarini Jr., J.R., Esquisatto, M.A.M., Mendona, J.S., Franchini, C.C., Santos, G.M.T., Effects of the application of Aloe vera (L.) and microcurrent on the healing of wounds surgically induced in Wistar rats (Rattus norvegicus) (2009) Acta Cirurgica Brasileira, 24 (1), pp. 150-155 Gill III, T.J., Smith, G.J., Wissler, R.W., Kunz, H.W., The rat as an experimental animal (1989) Science, 245 (4915), pp. 269-276 Frick, A., McCauley, D., Microcurrent electrical therapy (2005) Journal of Equine Veterinary Science, 25 (10), pp. 418-422. , DOI 10.1016/j.jevs.2005.09.012, PII S0737080605003886 Barker, A.T., Jaffe, L.F., Vanable Jr., J.W., The glabrous epidermis of cavies contains a powerful battery (1982) The American Journal of Physiology, 242 (3), pp. 358-366 Lee, B.Y., Al-Waili, N., Stubbs, D., Wendell, K., Butler, G., Al-Waili, T., Al-Waili, A., Ultra-low microcurrent in the management of diabetes mellitus, hypertension and chronic wounds: Report of twelve cases and discussion of mechanism of action (2010) International Journal of Medical Sciences, 7 (1), pp. 29-35 Anamika, M., Rasik, A.S., Antioxidant status in delayed healing type of wounds (2000) International Journal of Experimental Pathology, 81 (4), pp. 257-263 Carley, P.J., Wainapel, S.F., Electrotherapy for acceleration of wound healing: Low intensity direct current (1985) Archives of Physical Medicine and Rehabilitation, 66 (7), pp. 443-446 Nessler, J.P., Mass, D.P., Direct-current electrical stimulation of tendon healing in vitro (1987) Clinical Orthopaedics and Related Research, 217, pp. 303-312 Al-Waili, N., Electrotherapy for chronic gum and periapical abscesses (1989) Journal of the Pakistan Medical Association, 39 (6), pp. 161-162 Bassett, C.A.L., Beneficial effects of electromagnetic fields (1993) Journal of Cellular Biochemistry, 51 (4), pp. 387-393 Sheffet, A., Cytryn, A.S., Louria, D.B., Applying electric and electromagnetic energy as adjuvant treatment for pressure ulcers: A critical review (2000) Ostomy Wound Management, 46, pp. 28-33 Biedebach, M.C., Accelerated healing of skin ulcers by electrical stimulation and the intracellular physiological mechanisms involved (1989) Acupuncture and Electro-Therapeutics Research, 14 (1), pp. 43-60 Aaron, R.K., McK. Ciombor, D., Therapeutic effects of electromagnetic fields in the stimulation of connective tissue repair (1993) Journal of Cellular Biochemistry, 52 (1), pp. 42-46. , DOI 10.1002/jcb.240520107 MacEdo, A.C.B., Simes, N.D., Aplicao de estimulao elétrica de baixa intensidade no tratamento de lceras varicosas (2007) Fisioterapia em Movimento, 20 (3), pp. 23-33 Peruchi, C.M.S., Acevedo, R.A., Franco, S.L., Efecto del propleos en la cicatrizacin de lesiones subcutneas inducidas en el dorso del ratones: Estudio histolgico (2001) Revista de la Facultad de Odontologa de la Universidad de Chile, 19 (2), pp. 23-34 Ardisson, L., Godoy, J.S., Ferreira, L.A.M., Stehmann, J.R., Brando, M.G.L., Preparao e caracterizao de extratos gliclicos enriquecidos em taninos a partir das cascas de Stryphnodendron adstringens (Mart.) Coville (Barbatimo) (2002) Revista Brasileira de Farmacognosia, 12 (1), pp. 27-34 Bedi, M.K., Shenefelt, P.D., Herbal therapy in dermatology (2002) Archives of Dermatology, 138 (2), pp. 232-242 Lee, W.R., Park, J.H., Kim, K.H., Kim, S.J., Park, D.H., Chae, M.I.H., Suh, S.H., Park, K.K., The biological effects of topical alginate treatment in an animal model of skin wound healing (2009) Wound Repair and Regeneration, 17 (4), pp. 505-510 D'Acampora, A.J., Tramonte, R., Brger, D., Brger, P.J., Efeitos da quercetina na cicatrizao de ferida cirrgica contaminada em ratos Wistar (2007) Arquivos Catarinenses de Medicina, 36 (1), pp. 69-75 Arajo, T.A.S., Alencar, N.L., Amorim, E.L.C., Albuquerque, U.P., A new approach to study medicinal plants with tannins and flavonoids contents from the local knowledge (2008) Journal of Ethnopharmacology, 120 (1), pp. 72-80 Ermertcan, A.T., Inan, S., Ozturkcan, S., Bilac, C., Cilaker, S., Comparison of the effects of collagenase and extract of Centella asiatica in an experimental model of wound healing: An immunohistochemical and histopathological study (2008) Wound Repair and Regeneration, 16 (5), pp. 674-681 Fernandes, M.F.G., Oliveira, C.S., Mercadante-Simes, M.O., Mota, B.C.F., Aplicaes Teraputicas de Orquidceas III Frum Gesto, Pesquisa, Ensino e Extenso. Unimontes Universidade Estadual de Montes Claros, 2009 Fernandes, M.F.G., Oliveira, C.S., Mercadante-Simes, M.O., Mota, B.C.F., Aplicaes teraputicas de orquidceas (2009) III Frum Gesto, Pesquisa, Ensino e Extenso, , Montes Claros, Brazil Unimontes Universidade Estadual de Montes Claros Fiallo, V.R.F., Lorenzo, M.M.G., Herndez, C.M.L., Ferrad, C.A.R., Estudios fenolgicos en plantas medicinales (1998) Revista Cubana de Plantas Medicinais, 3 (1), pp. 7-11 Williams, C.A., Greenham, J., Harborne, J.B., Kong, J.-M., Chia, L.-S., Goh, N.-K., Saito, N., Tatsuzawa, F., Acylated anthocyanins and flavonols from purple flowers of Dendrobium cv. 'Pompadour' (2002) Biochemical Systematics and Ecology, 30 (7), pp. 667-675. , DOI 10.1016/S0305-1978(01)00147-8, PII S0305197801001478 Nayak, S.B., Sandiford, S., Maxwell, A., Evaluation of the wound-healing activity of ethanolic extract of Morinda citrifolia L. leaf (2009) Evidence-based Complementary and Alternative Medicine, 6 (3), pp. 351-356 Sntar, I.S., Koca, U., Akkol, E.K., Yilmazer, D., Alper, M., Assessment of wound healing activity of the aqueous extracts of Colutea cilicica Boiss. Bal. fruits and leaves (2011) Evidence-Based Complementary and Alternative Medicine, 2011, p. 7 Pesin, I., Koca, U., Keles, H., Akkol, E.K., Wound healing activity of Rubus sanctus Schreber (Rosaceae): Preclinical study in animal models Evidence-Based Complementary and Alternative Medicine, , In press Nayak, B.S., Suresh, R., Rao, A.V.C., Pillai, G.K., Davis, E.M., Ramkissoon, V., McRae, A., Evaluation of wound healing activity of Vanda roxburghii R.Br (Orchidacea): A preclinical study in a rat model (2005) International Journal of Lower Extremity Wounds, 4 (4), pp. 200-204. , DOI 10.1177/1534734605282994 Jorge Neto, J., Fracasso, J.F., Dos Santos, L.E., Banuth, V.L., Treatment of varicose ulcer and skin lesions with Calendula officinalis L. or Stryphnodendron barbadetiman (Vellozo) Martius (1996) Revista de Ciencias Farmaceuticas, 17, pp. 181-186 Soares, F.R.L., (2005) Reparao de Feridas Cutneas Tratadas Com Vitamina C, Laser e A Associao de Vitamina C e Laser: Estudo Histolgico em Ratos, M.S. Thesis, , Unimar Filho, A.L.M.M., Vilaverda, A.G.B., Arajo, K.S., Anlise do efeito do ultra-som teraputico associado ao Aloe vera no tratamento de tendinite experimental (2008) Proceedings of the 21th Congresso Brasileiro de Engenharia Biomédica, pp. 1359-1362 Mendona, G.B.N., Moraes, J.M., Ferreira, J., Laser As-Ga-Al de baixa potncia associado com soluo aquosa de barbatimo (Stryphynodendron barbatiman Martius) na reparao tecidual de ferida cutnea séptica de ovino, , Universidade Federal de Gois, 2008