Artículos de revistas
In Vitro Effect Of Low-level Laser On Odontoblast-like Cells
Registro en:
Laser Physics Letters. , v. 8, n. 2, p. 155 - 163, 2011.
16122011
10.1002/lapl.201010101
2-s2.0-78751700432
Autor
Oliveira C.F.
Basso F.G.
Lins E.C.
Kurachi C.
Hebling J.
Bagnato V.S.
De Souza Costa C.A.
Institución
Resumen
The aim of this study was to evaluate the metabolism of odontoblast-like MDPC-23 cells subjected to direct LLL irradiation. The cells were seeded (20,000 cells/well) in 24-well plates and incubated for 24 hours at 37°C. After this period, the culture medium (DMEM) was replaced by fresh DMEM supplemented with 2 or 5% (stress induction by nutritional deficit) or 10% fetal bovine serum (FBS). The cells were exposed to laser doses of 2, 4, 10, 15 and 25 J/cm2 from a near infrared InGaAsP diode laser prototype (LASERTable; 780±3 nm, 40 mW). One control group (sham irradiation) was established for each experimental condition (laser dose x FBS supplementation). Three and 72 hours after the last irradiation, cells were analyzed with respect to metabolism, morphology, total protein expression and alkaline phosphatase (ALP) activity. Higher metabolism and total protein expression were observed 72 hours after the last irradiation at the doses of 15 and 25 J/cm2 (Mann-Whitney; p<0.05). 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