Artículos de revistas
Partitioning Of Whey Proteins, Bovine Serum Albumin And Porcine Insulin In Aqueous Two-phase Systems
Registro en:
Journal Of Chromatography B: Biomedical Sciences And Applications. , v. 743, n. 01/02/15, p. 235 - 239, 2000.
13872273
10.1016/S0378-4347(00)00111-0
2-s2.0-0034705829
Autor
Alves J.G.L.F.
Chumpitaz L.D.A.
Da Silva L.H.M.
Franco T.T.
Meirelles A.J.A.
Institución
Resumen
Partitioning of the proteins from cheese whey, bovine serum albumin and porcine insulin were analysed using aqueous two-phase systems (ATPS) prepared with PEG-phosphate, PEG-citrate and PEG-maltodextrin (MD). Proteins were quantified through one of the following methods: FPLC, Bradford and spectrophotometry at 280 nm. Results showed that whey proteins partitioned unevenly on the phases of the systems used, with α-lactoalbumin (α-La) concentrated in the upper phase and β-lactoglobulin (β-Lg) in the lower. Albumin in PEG-MD systems concentrated in the MD-rich lower phase. Porcine insulin showed great affinity with the PEG-rich phase, its partition coefficient was always over 10 and increases with PEG molecular mass. (C) 2000 Elsevier Science B.V. 743 01/02/15 235 239 Diamond, A.D., Hsu, J.T., Aqueous two-phase systems for biomolecule separation (1992) Adv. Biochem. Eng.-Biotechnol., Berlin, 47, p. 90 Eiteman, M.A., Gainer, J.L., (1991) J. Chromatogr., 586, p. 341 Albertsson, P.A., Johansson, G., Tjerneld, F., (1990) Separation Processes in Biotechnology, , J.A. Asenjo. New York: Marcel Dekker Silva, L.H.M., Coimbra, J.S., Meirelles, A.J.A., (1997) J. Chem. Eng. Data, 42, p. 398 Zaslavsky, B.Y., (1995) Aqueous Two-Phase Partitioning, , New York: Marcel Dekker Duarte, M.C.T., Portugal, E.P., Ponezi, A.N., Bim, M.A., Tagliari, C.V., Franco, T.T., (1999) Biores. Techn., 68, p. 49 Bim, M.A., Franco, T.T., (1998) BIOTEC'98 Congress, , University of Minho-Guimarães, Portugal, 12-15 July Coimbra, J.S.R., Thommes, J., Meirelles, A.J.A., Kula, M.-R., (1995) Bioseparation, 5, p. 259 Coimbra, J.R., Thömes, J., Kula, M.-R., (1994) J. Chromatogr. a, 668, p. 85 Coimbra, J.S.R., Mojola, F., Meirelles, A.J.A., (1998) J. Chem. Eng. Japan, 31, p. 277 Sasakawa, S., Walter, H., (1972) Biochemistry, 15, p. 2760 Bradford, M.M., (1976) Anal. Biochem., 72, p. 248 Atkinson, L., Johns, M.R., (1994) Trans. I. Chem. E.: Part C, 72 Tanford, C., Epstein, J., (1954) J. Am. Chem. Soc., 76, p. 2163