Artículos de revistas
Tetracaine Stimulates Insulin Secretion Through The Mobilization Of Ca2+ From Thapsigargin- And Ip3-insensitive Ca2+ Reservoir In Pancreatic β-cells
Registro en:
Canadian Journal Of Physiology And Pharmacology. , v. 78, n. 6, p. 462 - 468, 2000.
84212
2-s2.0-0033922419
Autor
Bosqueiro J.R.
Carneiro E.M.
Bordin S.
Boschero A.C.
Institución
Resumen
The effect of tetracaine on 45Ca efflux, cytoplasmic Ca2+ concentration [Ca2+](i), and insulin secretion in isolated pancreatic islets and β-cells was studied. In the absence of external Ca2+, tetracaine (0.1-2.0 mM) increased the 45Ca efflux from isolated islets in a dose-dependant manner. Tetracaine did not affect the increase in 45Ca efflux caused by 50 mM K+ or by the association of carbachol (0.2 mM) and 50 mM K+. Tetracaine permanently increased the [Ca2+](i) in isolated β-cells in Ca2+-free medium enriched with 2.8 mM glucose and 25 μM D-600 (methoxiverapamil). This effect was also observed in the presence of 10 mM caffeine or 1 μM thapsigargin. In the presence of 16.7 mM glucose, tetracaine transiently increased the insulin secretion from islets perfused in the absence and presence of external Ca2+. These data indicate that tetracaine mobilises Ca2+ from a thapsigargin-insensitive store and stimulates insulin secretion in the absence of extracellular Ca2+. The increase in 45Ca efflux caused by high concentrations of K+ and by carbachol indicates that tetracaine did not interfere with a cation or inositol triphosphate sensitive Ca2+ pool in β-cells. 78 6 462 468 Aizawa, T., Sato, Y., Ishihara, F., Taguchi, N., Mitsuhisa, K., Suzuki, N., Hashizume, K., Yamada, Y., ATP-sensitive K+ channel-independent glucose action in rat pancreatic β-cell (1994) Am J. Physiol., 266, pp. C622-C627 Berridge, M.J., Elementary and global aspects of calcium signalling (1997) J. Physiol., 499, pp. 291-306 Bolaffi, J.L., Rodd, G.G., Ma, Y.H., Grodsky, G.M., Opposite, phase-dependent effects of 3,4,5-trimethoxybenzoic acid 8-(diethylamino) octyl ester or tetracaine on islets function during three phases of glucose-stimulated insulin secretion (1993) Endocrinology, 132, pp. 2325-2331 Bordin, S., Boschero, A.C., Carneiro, E.M., Atwater, I., Ionic mechanisms involved in the regulation of insulin secretion by muscarinic agonists (1995) J. Membr. Biol., 148, pp. 177-184 Bordin, S., Carneiro, E.M., Bosqueiro, J.R., Boschero, A.C., Tetracaine stimulates extracellular Ca2+-independent insulin release (1997) Eur. J. Pharmacol., 327, pp. 257-262 Boschero, A.C., Szpak-Glasman, M., Carneiro, E.M., Bordin, S., Paul, I., Rojas, E., Atwater, I., Oxotremorine-m potentiation of glucose-induced insulin release from rat islets involves M3 muscarinic receptors (1995) Am. J. Physiol., 268, pp. E336-E342 El Motal, S.M.A.A., Pian-Smith, M.C.M., Sharp, G.W.G., Effects of tetracaine on insulin release and calcium handling by rat pancreatic islets (1987) Am. J. Physiol., 252, pp. E727-E733 Freinkel, N., El Younsi, C., Dawson, R.M.C., Inter-relations between the phospholipids of rat pancreatic islets during glucose stimulation and their response to medium inositol and tetracaine (1975) Eur. J. Biochem., 59, pp. 245-252 Hellman, B., Gylfe, E., Bergsten, P., Grapengiesser, E., Lund, P.E., Berts, A., Dryselius, S., Chow, R.W., The role of Ca2+ in the release of pancreatic islet hormones (1994) Diabete Metab., 20, pp. 123-131 Hellman, B., Gylfe, E., Grapengiesser, E., Lund, P.E., Marcström, A., Cytoplasmic calcium and insulin secretion (1992) Nutrient Regulation of Insulin Secretion, pp. 213-245. , Edited by P. R. Flatt. Portland Press, London, U.K Herchuelz, A., Malaisse, W.J., Regulation of calcium fluxes in pancreatic islets: Two calcium movements' dissociated response to glucose (1980) Am. J. Physiol., 238, pp. E87-E95 Komatsu, M., Schermerhorn, T., Aizawa, T., Sharp, G.W.G., Glucose stimulation of insulin release in the absence of extracellular Ca2+ and in the absence of any increase in intracellular Ca2+ in rat pancreatic islets (1995) Proc. Natl. Acad. Sci. U.S.A., 92, pp. 10728-10732 Komatsu, M., Schermerhorn, T., Noda, M., Straub, S.G., Aizawa, T., Sharp, G.W.G., Augmentation of insulin release by glucose in the absence of extracellular Ca2+. New insights into stimulus-secretion coupling (1997) Diabetes, 46, pp. 1928-1938 Komatsu, M., Sharp, G.W.G., Aizawa, T., Hashizume, K., Glucose stimulation of insulin release without an increase in cytosolic free Ca2+ concentration: A possible involvement of GTP (1997) Jpn. J. Physiol., 47, pp. S22-S24 Komatsu, M., Noda, M., Sharp, G.W.G., Nutrient augmentation of Ca2+-dependent and Ca2+-independent pathways in stimulus-coupling to insulin secretion can be distinguished by their guanosine triphosphate requirements:studies on rat pancreatic islets (1998) Endocrinology, 139, pp. 1172-1183 Komatsu, M., Sharp, G.W.G., Palmitate and myristate selectively mimic the effect of glucose in augmenting insulin release in the absence of extracellular Ca2+ (1998) Diabetes, 47, pp. 352-357 Lacy, P.E., Kostianovsky, M., Method for the isolation of intact islets of Langerhans from the rat pancreas (1967) Diabetes, 16, pp. 35-39 Liu, Y.J., Tengholm, A., Grapengiesser, E., Hellman, B., Gylfe, E., Origin of slow and fast oscillations of Ca2+ in mouse pancreatic islets (1998) J. Physiol., 508, pp. 471-481 Low, P.S., Lloyd, D.H., Stein, T.M., Roger J.A. III, Calcium displacement by local anaesthetics. Dependence on pH and anaesthetic charge (1979) J. Biol. Chem., 254, pp. 4119-4125 Mears, D., Leighton, X., Atwater, I., Rojas, E., Tetracaine stimulates insulin secretion from the pancreatic β-cell by release of intracellular calcium (1999) Cell Calcium, 25, pp. 59-68 Nilsson, T., Arkhammar, P., Hallberg, A., Hellman, B., Berggren, P.-O., Characterisation of the inositol 1,4,5-triphosphate-induced Ca2+ release in pancreatic β-cells (1987) Biochem. J., 248, pp. 329-336 Norlund, L., Sehlin, J., Effect of tetracaine and lidocaine on insulin release in isolated mouse pancreatic islets (1983) Biochim. Biophys. Acta, 763, pp. 197-204 Nygren, P., Larsson, R., Lindh, E., Ljunghall, S., Ratad, J., Akerström, G., Gylfe, E., Bimodal regulation of secretion by cytosolic Ca2+ as evidenced by the parathyroid (1987) FEBS Lett., 213, pp. 195-198 Prentki, M., Matschinsky, F.M., Ca2+, cAMP, and phospholipid-derived messengers in coupling mechanisms of insulin secretion (1987) Physiol. Rev., 67, pp. 1185-1248 Rasmussen, H., Isales, C.M., Calle, R., Throckmorton, D., Anderson, M., Gasalla-Herraiz, J., McCarthy, R., Diacylglycerol production, Ca2+ influx, and protein kinase C activation in sustained cellular responses (1995) Endocrine Rev., 16, pp. 649-681 Sato, Y., Nenquin, M., Henquin, J.C., Relative contribution of Ca2+-dependent and Ca2+-independent mechanisms to the regulation of insulin secretion by glucose (1998) FEBS Lett., 421, pp. 115-119 Scott, A.M., Atwater, I., Rojas, E., A method for simultaneous measurement of insulin release and β-cell membrane potential in single mouse islets of langerhans (1981) Diabetologia, 21, pp. 470-475 Tengholm, A., Hagman, C., Gylfe, E., Hellman, B., In situ characterisation of nonmitochondrial Ca2+ stores in individual pancreatic β-cells (1998) Diabetes, 47, pp. 1224-1230 Van Eylen, F., Lebeau, C., Albuquerque-Silva, J., Herchuelz, A., Contribution of Na+ /Ca2+ exchange to Ca2+ outflow and entry in the rat pancreatic beta-cell. Studies with Antisense Oligonucleotides (1998) Diabetes, 47, pp. 1873-1880 Wollheim, C.B., Pozzan, T., Correlation between cytosolic free Ca2+ and insulin release in an insulin-secreting cell line (1984) J. Biol. Chem., 259, pp. 2262-2267