Actas de congresos
Adsorption Of Inulinases In Ion-exchange Columns
Registro en:
Applied Biochemistry And Biotechnology - Part A Enzyme Engineering And Biotechnology. , v. 84-86, n. , p. 1063 - 1078, 2000.
2732289
2-s2.0-0034036305
Autor
Silva F.-R.C.
Santana C.C.
Institución
Resumen
The use of adsorption columns packed with ion-exchange resins for recovering, concentrating and purifying proteins is now widespread. The present work consists of a study on the dynamic behavior of adsorption columns that uses two kinds of adsorbents: a cationic and an anionic resin. A frontal analysis of the columns was performed with experimental data obtained from Fructozyme, a mixture of inulinase enzymes. The parameters of a Langmuir type of isotherm and adsorption kinetics were obtained from experimental tests in a batch system. A numerical technique based on orthogonal collocation and a fourth-order Runge-Kutta method was coupled with a nonlinear optimization method to predict the coefficients of the rate equations, which are fundamental for scale-up purposes. 84-86
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